Papandrikopoulou A, Ender B E, Gassen H G
Biol Chem Hoppe Seyler. 1986 Sep;367(9):981-8. doi: 10.1515/bchm3.1986.367.2.981.
A new route for synthesis of a 32P-labeled Edman reagent 2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinane 2-oxide (PEPITC) is reported. The carrier-free [32P]-PEPITC (specific radioactivity 3.3 X 10(17) Bq/mol) in theory allows the labeling and detection of a minimum amount of 0.1 femtomol of protein. The advantages of the new reagent, which has previously been used for protein sequencing, are demonstrated by gelelectrophoretical and immunological experiments. Its major role is seen in the quantitative monitoring of protein elution from preparative one- and two-dimensional gels. Proteins thus obtained are, after the usual desalting steps, ready for sequencing.
报道了一种合成32P标记的埃德曼试剂2-(4-异硫氰酸苯氧基)-1,3,2-二氧磷杂环戊烷2-氧化物(PEPITC)的新路线。无载体的[32P]-PEPITC(比活度为3.3×10(17)Bq/mol)理论上允许标记和检测低至0.1飞摩尔的蛋白质。凝胶电泳和免疫学实验证明了这种先前用于蛋白质测序的新试剂的优点。其主要作用体现在对制备型一维和二维凝胶中蛋白质洗脱的定量监测上。经过常规脱盐步骤后，由此获得的蛋白质即可用于测序。
