聚丙烯酰胺凝胶上14C的自动扫描
Automated scanning of 14C on polyacrylamide gel.
作者信息
Magnusson R P, Jackiw A
出版信息
J Biochem Biophys Methods. 1979;1(1):65-8. doi: 10.1016/0165-022x(79)90047-2.
PMID:399485
Abstract
Cylindrical polyacrylamide gels were automatically analyzed for radioactive 14C using a commercially available radiochromatogram scanner. This method was capable of detecting peaks of [14C]protein containing 50 000--1 000 000 cpm. Under optimal conditions, gels could be scanned at a rate of 1 cm per min, allowing for analysis of 20--30 gels per day.
摘要
使用市售的放射性色谱扫描仪对圆柱形聚丙烯酰胺凝胶进行14C放射性自动分析。该方法能够检测出含有50000 - 1000000 cpm的[14C]蛋白质峰。在最佳条件下，凝胶可以以每分钟1厘米的速度进行扫描，每天可分析20 - 30块凝胶。
Zotero 插件