Hering Indra, Le Dang Tri, von Mikecz Anna
IUF - Leibniz Research Institute for Environmental Medicine.
EXCLI J. 2022 Jan 31;21:344-353. doi: 10.17179/excli2021-4626. eCollection 2022.
The global chemical inventory includes neurotoxins that are mostly interrogated concerning the biological response in developing organisms. Effects of pollutants on adults receive less attention, although vulnerabilities can be expected throughout the entire life span in young, middle-aged and old individuals. We use the animal model to systematically quantify neurological outcomes by application of an age-resolved method. Adult hermaphrodite worms were exposed to pollutants or non-chemical stressors such as temperature in liquid culture on microtiter plates and locomotion fitness was analyzed in a whole-life approach. Cultivation at 15, 20 or 25 °C showed that worms held at 15 °C displayed an enhanced level of fitness concerning swimming movements until middle age (11-days-old) and then a decline. In contrast, cultivated at ≥ 20 °C continually reduced their swimming movements with increasing age. Here, we provide a step-by-step protocol to investigate the health span of adult that may serve as a platform for automation and data collection. Consistent with this, more neurotoxins can be investigated with respect to vulnerable age-groups as well as contributing non-chemical environmental factors such as temperature.
全球化学物质清单中包含神经毒素，这些神经毒素大多是针对发育中的生物体的生物反应进行研究的。污染物对成年人的影响受到的关注较少，尽管在年轻人、中年人和老年人的整个生命周期中都可能存在易感性。我们使用动物模型，通过应用一种按年龄解析的方法来系统地量化神经学结果。将成年雌雄同体线虫暴露于污染物或非化学应激源（如微量滴定板液体培养中的温度）中，并采用全生命周期方法分析其运动适应性。在15、20或25°C下培养表明，在15°C下培养的线虫在中年（11日龄）之前游泳运动的适应性水平有所提高，然后下降。相比之下，在≥20°C下培养的线虫随着年龄的增长游泳运动持续减少。在此，我们提供了一个逐步方案，用于研究成年线虫的健康寿命，这可能为自动化和数据收集提供一个平台。与此一致的是，可以针对易受影响的年龄组以及温度等非化学环境因素来研究更多的神经毒素。
