Department of Orthopaedic Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Department of Preventive Medical Sciences, Fujita Health University School of Medicine, Toyoake, Japan.
Nagoya J Med Sci. 2022 Feb;84(1):60-68. doi: 10.18999/nagjms.84.1.60.
Frailty is a state of reduced muscle strength and activity in older people. DNA methylation is associated with osteoporosis and muscle loss in murine and other animal studies, but there are no epidemiological studies in humans. This study aimed to assess the association of osteoporosis and muscle loss with DNA methylation in community-dwelling older people. This cross-sectional study was performed in a rural part of Japan. We analyzed 204 subjects (98 men and 106 women). In univariate analysis, the two groups were compared according to the presence or absence of osteoporosis and of muscle loss. Logistic regression analysis was performed to determine predictors of frailty in the muscle loss group. We used age, sex, body mass index, smoking history, drinking history, serum albumin and C-reactive protein levels, diabetes, hypertension, hyperlipidemia, heart disease history, and LINE-1 DNA methylation as the factors. Probability values < 0.05 were considered to be statistically significant. The levels of LINE-1 DNA methylation in leukocytes were associated with muscle loss in men over the age of 60. LINE-1 DNA methylation levels were not associated with bone mineral density in either the men or women over the age of 60. LINE-1 DNA methylation levels in leukocytes correlated significantly with the risk of frailty in men over the age of 60. Promoting an understanding of DNA methylation may lead to a better understanding of the pathophysiology of muscle loss.
衰弱是老年人肌肉力量和活动减少的一种状态。DNA 甲基化与骨质疏松症和肌肉减少在鼠类和其他动物研究中相关，但在人类中尚无流行病学研究。本研究旨在评估社区居住的老年人中骨质疏松症和肌肉减少与 DNA 甲基化的相关性。这项横断面研究在日本农村地区进行。我们分析了 204 名受试者（98 名男性和 106 名女性）。在单因素分析中，根据是否存在骨质疏松症和肌肉减少，对两组进行了比较。对肌肉减少组进行逻辑回归分析，以确定衰弱的预测因素。我们将年龄、性别、体重指数、吸烟史、饮酒史、血清白蛋白和 C 反应蛋白水平、糖尿病、高血压、高脂血症、心脏病史以及 LINE-1 DNA 甲基化作为因素。概率值 < 0.05 被认为具有统计学意义。白细胞中 LINE-1 DNA 甲基化水平与 60 岁以上男性的肌肉减少有关。白细胞中 LINE-1 DNA 甲基化水平与 60 岁以上男性或女性的骨密度无关。白细胞中 LINE-1 DNA 甲基化水平与 60 岁以上男性发生衰弱的风险显著相关。对 DNA 甲基化的深入了解可能有助于更好地理解肌肉减少的病理生理学。
