Department of Orthopedic Surgery, Nagoya University Graduate School of Medicine, Aichi, Japan.
Clinical Laboratory Medicine, Fujita Health University Graduate School of Health Sciences, Aichi, Japan.
Biomed Res Int. 2020 Jul 30;2020:5047243. doi: 10.1155/2020/5047243. eCollection 2020.
Osteoporosis is a disease characterized by deterioration of bone tissue and mass, with an increasing global prevalence. Therefore, the discovery of biomarkers for osteoporosis would help to guide appropriate treatment. Circulating microRNAs (miRNAs) have become increasingly recognized as biomarkers for detecting diseases. However, few studies have investigated the association of circulating miRNA with osteoporosis in the general population. The aim of this study was to identify miRNA associated with osteoporosis in a general resident health check-up for potential use as an osteoporosis biomarker. We conducted a cross-sectional study as part of a health check-up program and recruited 352 volunteers (139 men, 213 women, mean age 64.1 ± 9.6 years). Osteoporosis was diagnosed according to the WHO classification. Twenty-two candidate microRNAs were screened through real-time quantitative PCR, and miRNAs associated with osteoporosis were analyzed using logistic regression analysis including other risk factors. In total, 95 females and 30 males were diagnosed with osteoporosis with bone mineral density tests (BMD: -score < -2.5). We found that miR195 was significantly lower in females, while miR150 and miR222 were significantly higher in males. The results of the logistic regression analysis indicated that in females, higher age and lower miR195 (odds ratio: 0.45, 95% confidential interval: 0.03-0.98) were significant risk factors for lower BMD, while the presence of a smoking habit and lower miR150 (odds ratio: 1.35, 95% confidential interval: 1.02-1.79) were significant risk factors for osteoporosis. Serum levels of miR195 and miR150 are independently associated with low bone mineral density in females and males, respectively.
骨质疏松症是一种以骨组织和骨量恶化为特征的疾病，其全球患病率正在不断上升。因此，发现骨质疏松症的生物标志物将有助于指导适当的治疗。循环 microRNAs（miRNAs）已越来越被认为是检测疾病的生物标志物。然而，很少有研究调查循环 miRNA 与普通人群中骨质疏松症的关联。本研究旨在通过一般居民健康检查中识别与骨质疏松症相关的 miRNA，以作为骨质疏松症生物标志物的潜在用途。我们进行了一项横断面研究，作为健康检查计划的一部分，招募了 352 名志愿者（男性 139 名，女性 213 名，平均年龄 64.1±9.6 岁）。骨质疏松症根据世界卫生组织分类进行诊断。通过实时定量 PCR 筛选了 22 种候选 microRNAs，并通过包括其他危险因素的逻辑回归分析分析了与骨质疏松症相关的 microRNAs。共有 95 名女性和 30 名男性被诊断为骨质疏松症，进行了骨矿物质密度测试（BMD：-score <-2.5）。我们发现，女性的 miR195 显著降低，而男性的 miR150 和 miR222 显著升高。逻辑回归分析的结果表明，在女性中，年龄较大和 miR195 较低（比值比：0.45，95%置信区间：0.03-0.98）是 BMD 降低的显著危险因素，而吸烟习惯和 miR150 降低（比值比：1.35，95%置信区间：1.02-1.79）是骨质疏松症的显著危险因素。血清 miR195 和 miR150 水平分别与女性和男性的低骨矿物质密度独立相关。
