Bourbon J R, Doucet E, Rieutort M
Biochim Biophys Acta. 1987 Jan 13;917(1):203-10. doi: 10.1016/0005-2760(87)90301-8.
The role of lysosomal enzyme acid alpha-glucosidase in fetal lung development was investigated with the aid of a specific inhibitor, the pseudosaccharide acarbose. The drug was added to a Waymouth culture medium of fetal rat lung explants cultivated for 48 h from gestational stage 19.5 days, an in vitro system previously shown to allow morphological and biochemical maturation of alveolar epithelium. Glycogenolysis was reduced by 40% as compared with tissue cultivated on control medium, which means that alpha-glucosidase could account for as much as 40% of fetal lung glycogenolysis, the remaining 60% being presumably achieved by cytosolic phosphorylase and by a microsomal neutral alpha-glucosidase. By the same time, the increase of phospholipids of surfactant fraction extracted from cultivated explants was partially inhibited: total and saturated phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were about 30-40% lower than in lungs cultivated on control medium. It should be emphasized that DNA concentration and increases in non-surfactant phospholipids were unchanged by the drug. alpha-Glucosidase activity was evidenced in the lysosomal fraction, in the microsomal fraction and, although in lower amounts, in the surfactant fraction extracted from term fetal lung. The results suggest that lysosomal alpha-glucosidase plays a major role in lung maturation and could facilitate glycogenolysis for the specific use of glycogen stores in providing substrates for surfactant phospholipid biosynthesis.
借助特异性抑制剂假糖阿卡波糖，研究了溶酶体酶酸性α-葡萄糖苷酶在胎儿肺发育中的作用。将该药物添加到从妊娠19.5天开始培养48小时的胎鼠肺外植体的Waymouth培养基中，此前已证明该体外系统可使肺泡上皮进行形态学和生化成熟。与在对照培养基上培养的组织相比，糖原分解减少了40%，这意味着α-葡萄糖苷酶可能占胎儿肺糖原分解的40%，其余60%可能由胞质磷酸化酶和微粒体中性α-葡萄糖苷酶完成。与此同时，从培养的外植体中提取的表面活性剂部分的磷脂增加受到部分抑制：总磷脂和饱和磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰肌醇比在对照培养基上培养的肺中低约30-40%。应该强调的是，药物对DNA浓度和非表面活性剂磷脂的增加没有影响。在溶酶体部分、微粒体部分以及从足月胎儿肺中提取的表面活性剂部分（尽管含量较低）中均证实了α-葡萄糖苷酶的活性。结果表明，溶酶体α-葡萄糖苷酶在肺成熟中起主要作用，并可能促进糖原分解，以便将糖原储备专门用于为表面活性剂磷脂生物合成提供底物。
