Piotrowicz B I, McCartney A C
Can J Microbiol. 1986 Oct;32(10):763-4. doi: 10.1139/m86-140.
Four modes of heating endotoxin in plasma and two different times of heating endotoxin in pyrogen-free water were compared. There were no significant differences in standard curves after heating endotoxin in plasma at 100 degrees C for 1 and for 10 min. However, the standard curve after heating for 10 min at 75 degrees C had a significantly less steep slope, and after heating for 10 min at 56 degrees C, it was completely flat. Heating of endotoxin in pyrogen-free water for 1 min also resulted in the flattening of the standard curve, which was even more pronounced after 10 min of heating.
比较了血浆中内毒素的四种加热模式以及无热原水中内毒素的两种不同加热时间。血浆中内毒素在100℃加热1分钟和10分钟后，标准曲线无显著差异。然而，在75℃加热10分钟后的标准曲线斜率明显更平缓，在56℃加热10分钟后，标准曲线完全平坦。无热原水中内毒素加热1分钟也导致标准曲线变平，加热10分钟后更为明显。
