McLean R J, Cheng K J, Gould W D, Nickel J C, Costerton J W
Can J Microbiol. 1986 Oct;32(10):772-8. doi: 10.1139/m86-142.
Proteus mirabilis, a gram-negative bacillus, is often implicated in the formation of infectious kidney stones. As ureolytic activity of this organism is thought to play a major role in its pathogenesis, we adapted our recently described urease localization technique to visualize urease activity in vivo. Urease activity was ultrastructurally localized in two clinically isolated P. mirabilis strains by precipitating the enzymatic reaction product (ammonia) with sodium tetraphenylboron. Subsequent silver staining of the cells revealed urease activity to be predominantly associated with the periplasm and outer membranes of each strain. Biochemical measurements of urease activity in P. mirabilis cell fractions correlated well with histochemical observations in that the majority of urease activity was associated with the periplasm. Membrane-bound urease activity of these strains was associated mainly with the peptidoglycan in the detergent-insoluble (outer membrane) fraction.
奇异变形杆菌是一种革兰氏阴性杆菌，常与感染性肾结石的形成有关。由于该菌的尿素分解活性被认为在其发病机制中起主要作用，我们采用了最近描述的尿素酶定位技术来观察体内的尿素酶活性。通过用四苯硼钠沉淀酶促反应产物（氨），在超微结构水平上定位了两种临床分离的奇异变形杆菌菌株中的尿素酶活性。随后对细胞进行银染，结果显示尿素酶活性主要与每个菌株的周质和外膜相关。奇异变形杆菌细胞组分中尿素酶活性的生化测量结果与组织化学观察结果高度相关，即大部分尿素酶活性与周质相关。这些菌株的膜结合尿素酶活性主要与去污剂不溶性（外膜）组分中的肽聚糖相关。
