Bode G, Malfertheiner P, Lehnhardt G, Nilius M, Ditschuneit H
Department of Internal Medicine I, University of Ulm, Germany.
Med Microbiol Immunol. 1993 Nov;182(5):233-42. doi: 10.1007/BF00579622.
Helicobacter pylori urease was characterized by means of an enzyme histochemical electron microscopic technique. Ultrastructural analysis revealed no urease activity in one strain; in seven H. pylori strains (43.75%), urease activity was associated with the cell membrane. Eight strains (50.0%) showed reaction product located within the cytoplasm. Urease activity showed no correlation with localization of activity. Our results demonstrate that H. pylori urease is not uniform in all H. pylori strains, and differences in activity and localization of urease activity may account for different virulence activities.
采用酶组织化学电子显微镜技术对幽门螺杆菌脲酶进行了表征。超微结构分析显示，一株菌株无脲酶活性；在7株幽门螺杆菌菌株（43.75%）中，脲酶活性与细胞膜相关。8株菌株（50.0%）的反应产物位于细胞质内。脲酶活性与活性定位无相关性。我们的结果表明，幽门螺杆菌脲酶在所有幽门螺杆菌菌株中并不一致，脲酶活性和定位的差异可能导致不同的毒力活性。
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