Stress and Oxidative Stress in Animal Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, 44000, Thailand.
Bioveterinary Research Unit, Faculty of Veterinary Sciences, Mahasarakham, 44000, Thailand.
J Therm Biol. 2022 Apr;105:103211. doi: 10.1016/j.jtherbio.2022.103211. Epub 2022 Feb 11.
The objective of this study was to examine the effect of increased ambient temperature on Hsp70 expression, superoxide dismutase (SOD) activity, nitric oxide (NO), malondialdehyde (MDA), caspase 8, caspase 9 and caspase 3 in relation to the intrinsic and extrinsic apoptosis pathway (IAP and EAP) of broiler blood cells (BBC). BBC were maintained at 42 °C and at 5 ranges of temperatures; 42-43, 44-45, 46-47, 48-49, and 50-51 °C. Then Hsp70 expression, SOD activity, NO, MDA, activity of caspase 8, caspase 9 and caspase 3, and living and apoptotic BBC were measured. It was found that Hsp70 expression and SOD were increased at 42-43 °C (P < 0.05), while the activity of caspase 9 and caspase 3 decreased (P < 0.05). At higher temperatures of 44-45 °C, caspase 9 and caspase 3 activities were increased and were higher than at 42 °C (P < 0.05). Apoptosis commenced in the temperature range 46-47 °C; during this range of temperature, NO increased (P < 0.05), but it decreased at 48-49 °C (P < 0.05). At the same time, activity of SOD in BBC was increased, achieving the highest levels (P < 0.05). In addition, activity of caspase 8 and caspase 3 at 48-49 °C was higher than that at 42 °C, and the apoptotic BBC was increased (P < 0.05). Therefore, temperature effects on BBC could be divided into three phases. The first phase was the temperature at 42 °C. The second phase, or pre-apoptotic phase, was in the temperature range from 42 to 45 °C. The third phase was the apoptosis phase, which manifested at 46 °C and above. These phenomena showed that in the pre-apoptosis phase, Hsp70 suppressed the IAP. On the other hand, in the apoptosis phase, apoptotic BBC is caused by the influence of NO, superoxide and MDA through the EAP.
本研究旨在探讨环境温度升高对肉鸡血细胞（broiler blood cells，BBC）中热休克蛋白 70（Hsp70）表达、超氧化物歧化酶（SOD）活性、一氧化氮（NO）、丙二醛（MDA）、半胱氨酸天冬氨酸蛋白酶 8（caspase 8）、半胱氨酸天冬氨酸蛋白酶 9（caspase 9）和半胱氨酸天冬氨酸蛋白酶 3（caspase 3）的影响，以及与内在和外在凋亡途径（intrinsic and extrinsic apoptosis pathway，IAP 和 EAP）的关系。将 BBC 在 42°C 下保持 5 个温度范围内，42-43°C、44-45°C、46-47°C、48-49°C 和 50-51°C。然后测量 Hsp70 表达、SOD 活性、NO、MDA、caspase 8、caspase 9 和 caspase 3 的活性，以及存活和凋亡的 BBC。结果发现，42-43°C 时 Hsp70 表达和 SOD 增加（P<0.05），而 caspase 9 和 caspase 3 的活性降低（P<0.05）。在 44-45°C 较高温度下，caspase 9 和 caspase 3 的活性增加，高于 42°C（P<0.05）。在 46-47°C 范围内，细胞凋亡开始；在此温度范围内，NO 增加（P<0.05），但在 48-49°C 时减少（P<0.05）。同时，BBC 中的 SOD 活性增加，达到最高水平（P<0.05）。此外，48-49°C 时 caspase 8 和 caspase 3 的活性高于 42°C，凋亡的 BBC 增加（P<0.05）。因此，温度对 BBC 的影响可分为三个阶段。第一阶段是 42°C 的温度。第二阶段，即凋亡前阶段，温度范围为 42-45°C。第三阶段是凋亡阶段，发生在 46°C 及以上。这些现象表明，在凋亡前阶段，Hsp70 抑制 IAP。另一方面，在凋亡阶段，通过 EAP，NO、超氧化物和 MDA 影响导致凋亡的 BBC。
