College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
J Sci Food Agric. 2018 Nov;98(14):5409-5417. doi: 10.1002/jsfa.9084. Epub 2018 Jun 10.
Resveratrol has been shown to prevent high ambient temperature (HT)-induced spleen dysplasia, but the mechanisms of action are not clear. This study aims to examine the hypothesis that HT-induced spleen dysplasia may be associated with HT-induced oxidative stress and apoptosis, and resveratrol may activate the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway, thus reducing oxidative stress and apoptosis.
Results showed that HT caused spleen dysplasia in broilers, reflecting the lower relative weight of the spleen (P < 0.05). Compared with birds in a normal ambient temperature group, birds in the HT group exhibited higher (P < 0.05) malondialdehyde (MDA), protein carbonyl (PC), 8-hydroxydeoxyguanosine (8-OHdG) and Bcl-2 associated X protein (Bax) content, higher Bax, caspase-3 and caspase-9 mRNA levels, and caspase-3 and caspase-9 activity, and a higher Bax/B-cell lympoma/leukemia-2 (Bcl-2) ratio, but they exhibited lower (P < 0.05) glutathione (GSH) and Bcl-2 content, and lower Nrf2, glutathione peroxidase (Gpx), MnSOD, heme oxygenase 1, glutathione reductase (GR) and Bcl-2 mRNA levels, and lower total antioxidant capacity (T-AOC), T-SOD and catalase and maganese superoixide dismutase (CAT) activity, indicating HT-induced oxidative stress and apoptosis. Compared with birds in the HT group, birds in the HT + Res group exhibited higher (P < 0.05) GSH and Bcl-2 content, higher Nrf2, CAT, MnSOD, GR and Bcl-2 mRNA levels, and higher T-AOC, T-SOD and CAT activity, but lower (P < 0.05) MDA content, and Bax and caspase-3 mRNA levels, lower caspase-3 and caspase-9 activities, and Bax/Bcl-2 ratio, indicating that resveratrol activated the Nrf2 signaling pathway and decreased apoptosis in the spleen.
Resveratrol was effective in ameliorating HT-induced spleen dysplasia in broilers through the activation of the Nrf2 signaling pathway, thereby decreasing apoptosis, suggesting that resveratrol may offer a potential nutritional strategy to protect against some HT-induced detriments. © 2018 Society of Chemical Industry.
白藜芦醇已被证明可预防高温环境（HT）引起的脾脏发育不良，但作用机制尚不清楚。本研究旨在检验以下假设，即 HT 引起的脾脏发育不良可能与 HT 引起的氧化应激和细胞凋亡有关，而白藜芦醇可能激活核因子红细胞 2 相关因子 2（Nrf2）信号通路，从而减少氧化应激和细胞凋亡。
结果表明，HT 导致肉鸡脾脏发育不良，反映脾脏相对重量较低（P<0.05）。与常温组相比，HT 组的丙二醛（MDA）、蛋白羰基（PC）、8-羟基脱氧鸟苷（8-OHdG）和 Bcl-2 相关 X 蛋白（Bax）含量较高（P<0.05），Bax、半胱天冬酶-3 和半胱天冬酶-9 mRNA 水平以及半胱天冬酶-3 和半胱天冬酶-9 活性较高，Bax/B 细胞淋巴瘤/白血病-2（Bcl-2）比值较高，但谷胱甘肽（GSH）和 Bcl-2 含量较低，Nrf2、谷胱甘肽过氧化物酶（Gpx）、MnSOD、血红素加氧酶 1、谷胱甘肽还原酶（GR）和 Bcl-2mRNA 水平较低，总抗氧化能力（T-AOC）、T-SOD 和过氧化氢酶和锰超氧化物歧化酶（CAT）活性较低，表明 HT 引起的氧化应激和细胞凋亡。与 HT 组相比，HT+Res 组的 GSH 和 Bcl-2 含量较高（P<0.05），Nrf2、CAT、MnSOD、GR 和 Bcl-2mRNA 水平较高，T-AOC、T-SOD 和 CAT 活性较高，但 MDA 含量较低，Bax 和半胱天冬酶-3mRNA 水平较低，半胱天冬酶-3 和半胱天冬酶-9 活性较低，Bax/Bcl-2 比值较低，表明白藜芦醇通过激活 Nrf2 信号通路减轻了肉鸡的 HT 诱导的脾脏发育不良，从而减少了细胞凋亡，这表明白藜芦醇可能提供了一种潜在的营养策略来预防一些 HT 引起的损害。© 2018 化学工业协会。
