Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE 68583, U.S.A.
Plant Stress and Germplasm Development Unit, Cropping Systems Research Laboratory, U.S. Department of Agriculture-Agricultural Research Service, Lubbock, TX 79415, U.S.A.
Mol Plant Microbe Interact. 2022 Sep;35(9):755-767. doi: 10.1094/MPMI-01-22-0005-R. Epub 2022 Sep 8.
The precursors and derivatives of jasmonic acid (JA) contribute to plant protective immunity to insect attack. However, the role of JA in sorghum () defense against sugarcane aphid (SCA) (), which is considered a major threat to sorghum production, remains elusive. Sorghum SC265, previously identified as a SCA-resistant genotype among the sorghum nested association mapping founder lines, transiently increased JA at early stages of aphid feeding and deterred aphid settling. Monitoring of aphid feeding behavior using electropenetrography, a technique to unveil feeding process of piercing-sucking insects, revealed that SC265 plants restricted SCA feeding from the phloem sap. However, exogenous application of JA attenuated the resistant phenotype and promoted improved aphid feeding and colonization on SC265 plants. This was further confirmed with sorghum JA-deficient plants, in which JA deficiency promoted aphid settling, however, it also reduced aphid feeding from the phloem sap and curtailed SCA population. Exogenous application of JA caused enhanced feeding and aphid proliferation on JA-deficient plants, suggesting that JA promotes aphid growth and development. SCA feeding on JA-deficient plants altered the sugar metabolism and enhanced the levels of fructose and trehalose compared with wild-type plants. Furthermore, aphid artificial diet containing fructose and trehalose curtailed aphid growth and reproduction. Our findings underscore a previously unknown dichotomous role of JA, which may have opposing effects by deterring aphid settling during the early stage and enhancing aphid proliferative capacity during later stages of aphid colonization on sorghum plants. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
茉莉酸（JA）的前体和衍生物有助于植物对昆虫攻击产生保护免疫。然而，JA 在高粱防御甘蔗蚜虫（SCA）（）中的作用仍不清楚，SCA 被认为是高粱生产的主要威胁。高粱 SC265 是高粱嵌套关联作图创始系中鉴定出的抗 SCA 基因型，它在蚜虫取食的早期阶段会短暂增加 JA，阻止蚜虫定殖。使用电穿透图（一种揭示刺吸式昆虫取食过程的技术）监测蚜虫的取食行为，发现 SC265 植物限制了 SCA 对韧皮部汁液的取食。然而，JA 的外源应用削弱了抗性表型，并促进了蚜虫在 SC265 植物上的取食和定殖。用 JA 缺乏的高粱植物进一步证实了这一点，在 JA 缺乏的植物中，JA 缺乏促进了蚜虫的定殖，但也减少了蚜虫对韧皮部汁液的取食，并抑制了 SCA 种群。JA 的外源应用导致 JA 缺乏的植物上的取食和蚜虫增殖增强，表明 JA 促进了蚜虫的生长和发育。与野生型植物相比，SCA 在 JA 缺乏的植物上取食会改变糖代谢，提高果糖和海藻糖的水平。此外，含有果糖和海藻糖的蚜虫人工饲料会抑制蚜虫的生长和繁殖。我们的研究结果强调了 JA 的一个以前未知的二分角色，它可能通过在蚜虫早期定殖阶段阻止定殖，以及在蚜虫后期定殖阶段增强蚜虫增殖能力，从而产生相反的作用。[公式：见正文] 版权所有 © 2022 作者。这是一份在 CC BY-NC-ND 4.0 国际许可下分发的开放获取文章。
