评价不同玫瑰品种对蚜虫的抗性及抗蚜过程中的转录组分析。

Evaluation of aphid resistance on different rose cultivars and transcriptome analysis in response to aphid infestation.

机构信息

Hebei Key Laboratory of Plant Genetic Engineering, Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050051, China.

Department of Ornamental Horticulture, State Key Laboratory of Agrobiotechnology, Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, College of Horticulture, China Agricultural University, Beijing, 100193, China.

出版信息

BMC Genomics. 2024 Mar 4;25(1):232. doi: 10.1186/s12864-024-10100-z.

DOI:10.1186/s12864-024-10100-z

PMID:38438880

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10910744/

Abstract

BACKGROUND

The rose is one of the most important ornamental flowers in the world for its aesthetic beauty but can be attacked by many pests such as aphids. Aphid infestation causes tremendous damage on plant tissues leading to harmed petals and leaves. Rose cultivars express different levels of resistance to aphid infestation yet the information remains unclear. Not only that, studies about the transcriptional analysis on defending mechanisms against aphids in rose are limited so far.

RESULTS

In this study, the aphid resistance of 20 rose cultivars was evaluated, and they could be sorted into six levels based on the number ratio of aphids. And then, a transcriptome analysis was conducted after aphid infestation in one high resistance (R, Harmonie) and one highly susceptibility (S, Carefree Wonder) rose cultivar. In open environment the majority of rose cultivars had the highest aphid number at May 6th or May 15th in 2020 and the resistance to infestation could be classified into six levels. Differential expression analysis revealed that there were 1,626 upregulated and 767 downregulated genes in the R cultivar and 481 upregulated and 63 downregulated genes in the S cultivar after aphid infestation. Pathway enrichment analysis of the differentially expressed genes revealed that upregulated genes in R and S cultivars were both enriched in defense response, biosynthesis of secondary metabolites (phenylpropanoid, alkaloid, and flavonoid), carbohydrate metabolism (galactose, starch, and sucrose metabolism) and lipid processing (alpha-linolenic acid and linolenic acid metabolism) pathways. In the jasmonic acid metabolic pathway, linoleate 13S-lipoxygenase was specifically upregulated in the R cultivar, while genes encoding other crucial enzymes, allene oxide synthase, allene oxide cyclase, and 12-oxophytodienoate reductase were upregulated in both cultivars. Transcription factor analysis and transcription factor binding search showed that WRKY transcription factors play a pivotal role during aphid infestation in the R cultivar.

CONCLUSIONS

Our study indicated the potential roles of jasmonic acid metabolism and WRKY transcription factors during aphid resistance in rose, providing clues for future research.

摘要

背景

玫瑰因其美学之美，是世界上最重要的观赏花卉之一，但也会受到许多害虫的侵袭，如蚜虫。蚜虫的侵害会对植物组织造成巨大的损害，导致花瓣和叶片受损。不同的玫瑰品种对蚜虫的侵害表现出不同程度的抗性，但信息仍然不清楚。不仅如此，目前关于玫瑰抵御蚜虫的转录分析研究还很有限。

结果

本研究评估了 20 个玫瑰品种的抗蚜性，根据蚜虫数量比值将其分为六个等级。然后，在一个高抗（R，Harmonie）和一个高感（S，Carefree Wonder）玫瑰品种被蚜虫侵害后进行转录组分析。在开放环境中，大多数玫瑰品种在 2020 年 5 月 6 日或 5 月 15 日蚜虫数量最多，抗虫性可分为六个等级。差异表达分析显示，在 R 品种中，有 1626 个上调基因和 767 个下调基因，在 S 品种中有 481 个上调基因和 63 个下调基因在蚜虫侵害后表达。差异表达基因的通路富集分析显示，R 和 S 品种的上调基因均富集在防御反应、次生代谢物（苯丙烷、生物碱和类黄酮）合成、碳水化合物代谢（半乳糖、淀粉和蔗糖代谢）和脂质加工（亚麻酸和亚油酸代谢）途径中。在茉莉酸代谢途径中，亚油酸 13S-脂氧合酶在 R 品种中特异性上调，而其他关键酶，如烯氧合酶、烯氧环化酶和 12-氧代植二烯酸还原酶在两个品种中均上调。转录因子分析和转录因子结合搜索显示，WRKY 转录因子在 R 品种蚜虫侵害过程中发挥着关键作用。