Poli F, Pancaldi S, Vannini G L
Eur J Cell Biol. 1986 Oct;42(1):79-83.
Monensin, a monovalent cation ionophore, was used to investigate some steps of the wall synthesis and morphogenesis in Candida albicans blastospores. In the presence of the drug, the pathogenic yeast developed enormous wall and septum thickenings that reacted intensely and specifically with wheat germ agglutinin and chitinase coupled to colloidal gold and fluorescein isothiocyanate. Therefore, the aberrant zones are interpreted as sites of chitin accumulation. The increased production of this homopolymer, also demonstrated by the chemical analysis of cell wall preparations, implies that monensin interferes in some way with the regulatory factors that normally control, in space and time, chitin synthetase activity.
莫能菌素是一种单价阳离子离子载体，被用于研究白色念珠菌芽生孢子细胞壁合成和形态发生的某些步骤。在该药物存在的情况下，致病酵母形成了巨大的细胞壁和隔膜增厚，这些增厚区域与偶联胶体金和异硫氰酸荧光素的麦胚凝集素及几丁质酶发生强烈且特异性反应。因此，这些异常区域被解释为几丁质积累的部位。通过对细胞壁制剂的化学分析也证实了这种同聚物产量的增加，这意味着莫能菌素以某种方式干扰了通常在空间和时间上控制几丁质合成酶活性的调节因子。
