Hilenski L L, Naider F, Becker J M
J Gen Microbiol. 1986 Jun;132(6):1441-51. doi: 10.1099/00221287-132-6-1441.
Yeasts and mycelia of the pathogen Candida albicans grown in the presence of polyoxin D, a competitive inhibitor of chitin synthase, formed chains of swollen bulbous cells as observed by fluorescence microscopy. Wheat germ agglutinin (WGA) complexed to colloidal gold (Au) was used as a specific label at the ultrastructural level to visualize chitin in walls of control and polyoxin-treated cells. In control cells, Au-WGA labelling was preferentially localized in the innermost wall layers and was predominant at bud scars and septa. After 4.5 h in 4 mM-polyoxin D, budding in yeasts and lateral wall growth in mycelia continued, but primary septa failed to form and no Au-WGA labelling was detected in the walls. These results demonstrated that the morphological alterations caused by polyoxin D were due to the absence of chitin, a wall component important for formation of primary septa and for maintenance of structural integrity during morphogenesis.
在几丁质合成酶的竞争性抑制剂多氧霉素D存在的情况下生长的病原体白色念珠菌的酵母和菌丝体，通过荧光显微镜观察到形成了肿胀的球状细胞链。与胶体金（Au）复合的小麦胚凝集素（WGA）在超微结构水平用作特异性标记，以可视化对照细胞和经多氧霉素处理的细胞壁中的几丁质。在对照细胞中，金-小麦胚凝集素标记优先定位在最内层壁层，并且在芽痕和隔膜处占主导。在4 mM多氧霉素D中培养4.5小时后，酵母中的出芽和菌丝体中的侧壁生长继续，但初级隔膜未能形成，并且在壁中未检测到金-小麦胚凝集素标记。这些结果表明，多氧霉素D引起的形态学改变是由于几丁质的缺失，几丁质是一种对初级隔膜形成和形态发生过程中结构完整性维持很重要的壁成分。
