Pancaldi S, Bruni A, Dall'Olio G, Fasulo M P
Institute of Botany, University of Ferrara, Italy.
Microbios. 1994;79(320):163-70.
Monensin, an exocytosis perturber, was assayed on the dimorphic fungus Candida albicans in order to study its effects on wall synthesis and morphogenesis. In the presence of the drug, both germ-tube formation and hyphal elongation were markedly inhibited according to the dose and exposure time. The effect was accompanied by morphogenetic malformations which could be detected under UV light and by transmission electron microscopy. Enormous wall and septa thickenings revealed by cytochemical tests indicated an abnormal deposition of chitin. Since chitin synthase is inserted in the plasma membrane in a latent state and its activation depends on regulatory factors reaching the cell periphery through an orderly exocytosis, it is assumed that monensin affects the vesicular traffic leading to a prevalence of activators over inhibitors of the enzyme.
莫能菌素是一种胞吐作用干扰剂，为了研究其对细胞壁合成和形态发生的影响，对二态真菌白色念珠菌进行了检测。在药物存在的情况下，根据剂量和暴露时间，芽管形成和菌丝伸长均受到明显抑制。这种作用伴随着形态发生畸形，可在紫外光下和通过透射电子显微镜检测到。细胞化学试验显示的巨大细胞壁和隔膜增厚表明几丁质沉积异常。由于几丁质合酶以潜伏状态插入质膜，其激活取决于通过有序胞吐作用到达细胞周边的调节因子，因此推测莫能菌素影响囊泡运输，导致该酶的激活剂比抑制剂占优势。
