School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou, PR China.
Centre for Tactile Internet with Human-in-the-Loop (CeTI), Technische Universität Dresden, 01069, Dresden, Germany.
J Sci Food Agric. 2022 Oct;102(13):5729-5737. doi: 10.1002/jsfa.11922. Epub 2022 Apr 23.
The bioavailability of curcumin (Cur) is generally limited by its poor stability. However, it is beneficial to improve the stability of Cur by using self-assembled zein hydrolysate (ZH) as delivery carrier. This paper aimed to explore the formation mechanism of zein hydrolysate-curcumin nanocomplexes as a function of critical micelle concentration (CMC).
In this work, The CMC of ZH (0.535 mg mL ) was obtained by the pyrene fluorescent probe method. ZH-Cur nanocomplexes undergo hydrogen bonding and hydrophobic interactions, and the fluorescence quenching effect was concentration dependent with the process of static quenching. Moreover, the differences of colloidal properties on ZH and ZH-Cur nanocomplexes were systematically compared by dynamic light scattering and scanning electron microscopy near CMC. ZH presented irregular spherical shapes and would aggregate to form micelles at the CMC and above. The tight micellar structure promoted more uniform size distribution (double peaks reduced) and higher potentials (over -30 mV) within 10 days. In addition, the nanocomplexes demonstrated an obvious core-shell structure. Within 10 days of storage, the particle size distributions were uniform and the potentials increased significantly, indicating that the micellar nanostructure made the Cur stably embedded in the hydrophobic core of ZH. Finally, ZH-Cur nanocomplexes effectively improved the water solubility and encapsulation rate (over 70%) of Cur. Moreover, over 90% of Cur was released steadily within 91 h.
This work provided a theoretical basis for the application of amphiphilic peptide micellar nanostructure as novel food-grade nanocarriers to transport hydrophobic bioactive substances. © 2022 Society of Chemical Industry.
姜黄素（Cur）的生物利用度通常受到其较差的稳定性的限制。然而，通过使用自组装的玉米醇溶蛋白水解物（ZH）作为递送载体，可以有利于提高 Cur 的稳定性。本文旨在探索作为临界胶束浓度（CMC）函数的玉米醇溶蛋白水解物-姜黄素纳米复合物的形成机制。
在这项工作中，通过芘荧光探针法获得了 ZH 的 CMC（0.535mgmL-1）。ZH-Cur 纳米复合物通过氢键和疏水相互作用形成，荧光猝灭效应与静态猝灭过程呈浓度依赖性。此外，在 CMC 附近，通过动态光散射和扫描电子显微镜系统地比较了 ZH 和 ZH-Cur 纳米复合物的胶体性质差异。ZH 呈现不规则的球形，在 CMC 及以上会聚集形成胶束。紧密的胶束结构促进了更均匀的粒径分布（双峰减少）和更高的电位（超过-30mV），在 10 天内。此外，纳米复合物表现出明显的核壳结构。在 10 天的储存期内，粒径分布均匀，电位显著增加，表明胶束纳米结构使 Cur 稳定地嵌入 ZH 的疏水核中。最后，ZH-Cur 纳米复合物有效提高了 Cur 的水溶性和包封率（超过 70%）。此外，超过 90%的 Cur 在 91h 内稳定释放。
这项工作为应用两亲肽胶束纳米结构作为新型食品级纳米载体来输送疏水性生物活性物质提供了理论依据。© 2022 化学工业协会。
