Shanghai Engineering Research Center of Food Microbiology, School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Food Funct. 2024 Mar 4;15(5):2563-2576. doi: 10.1039/d3fo04594c.
In this work, a zein-tamarind seed polysaccharide (TSP) co-delivery system was fabricated using an anti-solvent precipitation method. The formation mechanism, characterization, and effect on alleviating colitis and gut microbiota dysbiosis in mice of zein-TSP-curcumin (Z/T-Cur) nanocomplexes were investigated. Hydrogen bonding and the hydrophobic effect played a key role in the formation of Z/T-Cur nanocomplexes, and the interactions were spontaneous and driven by enthalpy. The encapsulation efficiency, loading capacity, and bioavailability increased from 60.8% (Zein-Cur) to 91.7% (Z/T-Cur), from 6.1% (Zein-Cur) to 18.3% (Z/T-Cur), and from 4.7% (Zein-Cur) to 20.0% (Z/T-Cur), respectively. The Z/T-Cur significantly alleviated colitis symptoms in DSS-treated mice. Additionally, the prepared nanocomplexes rebalanced the gut microbiota composition of colitis mice by increasing the abundance of . and were rich in the Z-T-Cur treatment group, and and were rich in the zein-TSP treatment group. This study demonstrated that the TSP could be helpful in the targeted drug delivery system.
在这项工作中，采用抗溶剂沉淀法制备了玉米醇溶蛋白-罗望子多糖（TSP）共递送系统。研究了玉米醇溶蛋白-TSP-姜黄素（Z/T-Cur）纳米复合物的形成机制、表征以及缓解结肠炎和肠道微生物失调的作用。氢键和疏水作用在 Z/T-Cur 纳米复合物的形成中起关键作用，相互作用是自发的，由焓驱动。包封效率、载药量和生物利用度从 60.8%（玉米醇溶蛋白-姜黄素）增加到 91.7%（Z/T-Cur），从 6.1%（玉米醇溶蛋白-姜黄素）增加到 18.3%（Z/T-Cur），从 4.7%（玉米醇溶蛋白-姜黄素）增加到 20.0%（Z/T-Cur）。Z/T-Cur 显著缓解了 DSS 处理的结肠炎小鼠的症状。此外，所制备的纳米复合物通过增加. 的丰度来重新平衡结肠炎小鼠的肠道微生物群落组成。在 Z/T-Cur 处理组中， 和 丰富，在玉米醇溶蛋白-TSP 处理组中， 和 丰富。这项研究表明，TSP 可以在靶向药物传递系统中发挥作用。
