Department of Periodontology/Implantology, Universidade Federal de Uberlândia - UFU, School of Dentistry, Uberlândia, MG, Brazil.
Department of Diagnosis and Surgery, Universidade Estadual Paulista - UNESP, School of Dentistry, Araraquara, SP, Brazil.
Microsc Res Tech. 2022 Aug;85(8):2776-2783. doi: 10.1002/jemt.24126. Epub 2022 Apr 9.
This study evaluated the osseointegration of implants in areas grafted with biphasic ceramic based on hydroxyapatite/β-tricalcium phosphate (HA/TCP) and in native bone (NB). Twenty-eight rats were randomly assigned into two groups of 14 animals each: HA/TCP group: implants installed in areas grafted with HA/TCP and NB group: implants installed in areas of native bone. Bone defects were made in both tibiae of the rats belonging to the HA/TCP group and then filled with this bone substitute. After 60 days, the rats were submitted to surgical procedures for implant placement in grafted areas in both tibiae in the HA/TCP group while the implants were installed directly in native bone in the NB group. The animals were euthanized 15 and 45 days, respectively, after the implant placement. Biomechanical (removal torque), microtomographic (volume of mineralized tissues around the implants), and histomorphometric (Bone-Implant contact-%BIC and bone area between the implant threads-%BBT) analyzes were conducted to assess the osseointegration process. The HA/TCP group showed lower values of removal torque, volume of mineralized tissue around the implants, lower %BIC, and %BBT compared to the NB group in both experimental periods. Osseointegration of implants placed in grafted areas with HA/TCP was lower compared to the osseointegration observed in native bone areas. RESEARCH HIGHLIGHTS: The areas grated with HA/TCP presented poor biological conditions. The reduced biological properties for bone formation impaired the osseointegration in HA/TCP grafted areas.
本研究评估了基于羟基磷灰石/β-磷酸三钙（HA/TCP）的双相陶瓷骨移植区和自体骨（NB）中植入物的骨整合情况。将 28 只大鼠随机分为两组，每组 14 只：HA/TCP 组：植入物植入 HA/TCP 移植区；NB 组：植入物植入自体骨区。HA/TCP 组大鼠双侧胫骨均制作骨缺损，并用该骨替代物填充。60 天后，HA/TCP 组大鼠双侧胫骨移植区均行植入物植入手术，NB 组大鼠直接在自体骨中植入植入物。植入物植入后 15 天和 45 天，分别处死大鼠。进行生物力学（去除扭矩）、微断层扫描（植入物周围矿化组织体积）和组织形态计量学（骨-植入物接触-%BIC 和植入物螺纹之间的骨面积-%BBT）分析，以评估骨整合过程。在两个实验期，HA/TCP 组的去除扭矩、植入物周围矿化组织体积、%BIC 和 %BBT 值均低于 NB 组。与自体骨区相比，HA/TCP 移植区植入物的骨整合较低。研究亮点：HA/TCP 移植区的生物学条件较差。骨形成的生物特性降低，影响了 HA/TCP 移植区的骨整合。
