de Oliveira Guilherme José Pimentel Lopes, Aroni Maurício Andres Tinajero, Pinotti Felipe Eduardo, Marcantonio Elcio, Marcantonio Rosemary Adriana Chiérici
Department of Periodontology, Dental School, Universidade Federal de Uberlândia (UFU), Pará Av, 1720, Uberlândia, MG, 38400-902, Brazil.
Department of Diagnosis and Surgery, School of Dentistry Campus of Araraquara, São Paulo State University (UNESP), Humaitá St. 1680, Araraquara, 14801-903, Brazil.
Lasers Med Sci. 2020 Sep;35(7):1519-1529. doi: 10.1007/s10103-019-02943-w. Epub 2020 Feb 5.
The aim of this study was to evaluate the osseointegration of implants placed in areas grafted with different osteoconductive bone substitutes irradiated with infrared low-level laser therapy (LLLT). Fifty-six rats were randomly allocated into 4 groups: DBB, bone defects filled with deproteinized bovine bone graft (DBB); HA/TCP, bone defects filled with biphasic ceramic made of hydroxyapatite and β-tricalcium phosphate (HA/TCP); DBB-L, bone defects filled with DBB and treated by LLLT; HA/TCP-L, bone defects filled with HA/TCP and treated by LLLT. Bone defects were performed in the tibia of each animal and filled with the different biomaterials. The grafted areas were treated with LLLT (λ 808 nm, 100 mW, ϕ ∼ 0.60 mm) in 7 sessions with 48 h between the irradiations. After the 60-day period, the implants were placed, and the animals were euthanized after 15 and 45 days. The osseointegration and bone repair in the grafted area were evaluated by biomechanical, microtomographic and histometric analyses, and the expression of some bone biomarkers was evaluated by immunohistochemistry analysis. LLLT induced higher degree of osseointegration, which was associated with the greater expression of BMP2 and OCN. LLLT performed in areas grafted with osteoconductive bone substitutes prior to implant placement improves osseointegration.
本研究的目的是评估在植入不同骨传导性骨替代物并接受红外低强度激光治疗（LLLT）的区域中种植体的骨整合情况。56只大鼠被随机分为4组：DBB组，骨缺损填充脱蛋白牛骨移植材料（DBB）；HA/TCP组，骨缺损填充由羟基磷灰石和β-磷酸三钙制成的双相陶瓷（HA/TCP）；DBB-L组，骨缺损填充DBB并接受LLLT治疗；HA/TCP-L组，骨缺损填充HA/TCP并接受LLLT治疗。在每只动物的胫骨上制造骨缺损，并用不同的生物材料填充。移植区域接受LLLT治疗（波长808 nm，100 mW，光斑直径约0.60 mm），共7次，每次照射间隔48小时。60天后植入种植体，15天和45天后对动物实施安乐死。通过生物力学、显微断层扫描和组织计量学分析评估移植区域的骨整合和骨修复情况，并通过免疫组织化学分析评估一些骨生物标志物的表达。LLLT诱导了更高程度的骨整合，这与BMP2和OCN的更高表达相关。在植入种植体之前，对植入骨传导性骨替代物的区域进行LLLT可改善骨整合。
