Chen Yi-Jyun, Hsu Ying-Pei, Tain You-Lin, Li Nan-Si, Pang Hao-Han, Kuo Shiao-Wei, Yang Hung-Wei
Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan.
Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan; Department of Materials and Optoelectronic Science, Center for Functional Polymers and Supramolecular Materials, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
Biosens Bioelectron. 2022 Jul 15;208:114234. doi: 10.1016/j.bios.2022.114234. Epub 2022 Mar 31.
Chronic kidney disease (CKD) is the most neglected chronic disease affecting over 750 million persons in the world. Currently, many patients with cancers or other chronic diseases (i.e., CKD) struggle to receive clinical treatment or examination due to hospitals cancelling or delaying in the COVID-19 pandemic, which may increase the risk of death. Cystatin C (Cys C) has been proposed as a potential glomerular filtration rate (GFR) marker for the early detection of acute kidney injury and CKD. However, most traditional methods for Cys C detection are immunoassays using serum as a sample and are tedious to perform and economically burdensome. To diagnose the disease in the early stage and carry out daily management during the current pandemic, we developed an integration of hydrogel microneedle patch (HMNP) and lateral flow cassette (LFC) to rapidly detect Cys C in skin interstitial fluid (ISF) in 25 min for blood-free CKD management anytime and anywhere by the naked eye that can reduce the impact of an individual's quality of life and life expectancy. Conceivably, this strategy presents a wide scope in the application of numerous other diseases if corresponding analytes are available in skin ISF.
慢性肾脏病（CKD）是世界上最被忽视的慢性疾病，影响着超过7.5亿人。目前，由于医院在新冠疫情期间取消或推迟治疗，许多癌症或其他慢性疾病（如CKD）患者难以接受临床治疗或检查，这可能会增加死亡风险。胱抑素C（Cys C）已被提议作为一种潜在的肾小球滤过率（GFR）标志物，用于早期检测急性肾损伤和CKD。然而，大多数传统的Cys C检测方法是使用血清作为样本的免疫测定法，操作繁琐且成本高昂。为了在当前疫情期间早期诊断疾病并进行日常管理，我们开发了一种水凝胶微针贴片（HMNP）和侧流检测盒（LFC）的集成装置，可在25分钟内快速检测皮肤间质液（ISF）中的Cys C，实现随时随地肉眼无血检测CKD，从而减少对个人生活质量和预期寿命的影响。可以想象，如果皮肤ISF中存在相应的分析物，这种策略在众多其他疾病的应用中具有广泛的前景。
