"Iuliu Hațieganu" University of Medicine and Pharmacy, Department of Analytical Chemistry, 4 Pasteur St., 400349, Cluj-Napoca, Romania.
"Iuliu Hațieganu" University of Medicine and Pharmacy, Department of Analytical Chemistry, 4 Pasteur St., 400349, Cluj-Napoca, Romania.
Anal Chim Acta. 2022 Apr 29;1204:339696. doi: 10.1016/j.aca.2022.339696. Epub 2022 Mar 8.
The recent increase in outbreaks of pathogenic bacteria and antimicrobial resistance represent major public health problems. Being the leading cause of death in humans, the bacterial infections need to be accurately and quickly diagnosed. Hence, the development of fast, cost-effective, sensitive and specific strategies for the detection of the targeted bacterium is of utmost importance. This review presents a systematic, critical evaluation of the recent analytical methods for the characterization and diagnosis of infections caused by Pseudomonas aeruginosa. The clinical manifestations, incidence and treatment of the P. aeruginosa infection and the associated quorum sensing, biofilm formation and virulence factors are also discussed. An overview of a variety of analytical methods for the detection of P. aeruginosa is provided, including whole-cell detection (microbiological methods, biosensors), antigens, DNA, and relevant markers (quorum sensing molecules, virulence factors) detection. The latest trends in analytical methods, especially sensors, are the orientation towards portability and on-site detection. The efforts made so far to achieve these goals in the detection of P. aeruginosa and its markers are also presented and discussed in this review. The strengths and weaknesses of the current detection methods are evaluated, while exploring potential routes for further development.
近年来，致病性细菌和抗微生物药物耐药性的爆发增加，这是主要的公共卫生问题。细菌感染是人类死亡的主要原因，因此需要准确、快速地诊断。因此，开发快速、经济有效、灵敏和特异的靶向细菌检测策略至关重要。本综述系统地、批判性地评价了近年来用于鉴定和诊断铜绿假单胞菌感染的分析方法。本文还讨论了铜绿假单胞菌感染的临床表现、发生率和治疗方法以及群体感应、生物膜形成和毒力因子。综述了各种用于检测铜绿假单胞菌的分析方法，包括全细胞检测（微生物方法、生物传感器）、抗原、DNA 和相关标志物（群体感应分子、毒力因子）检测。本综述还介绍和讨论了分析方法（尤其是传感器）在便携性和现场检测方面的最新趋势。本文还探讨了在检测铜绿假单胞菌及其标志物方面为实现这些目标所做的努力，并对当前检测方法的优缺点进行了评估，同时探讨了进一步发展的潜在途径。
