Frigoli Margaux, Lowdon Joseph W, Donetti Nicolas, Crapnell Robert D, Banks Craig E, Cleij Thomas J, Diliën Hanne, Eersels Kasper, van Grinsven Bart
Sensor Engineering Department, Faculty of Science and Engineering, Maastricht University, P.O. Box 616, Maastricht 6200 MD, The Netherlands.
John Dalton Building, Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, U.K.
ACS Omega. 2024 Aug 14;9(34):36411-36420. doi: 10.1021/acsomega.4c03970. eCollection 2024 Aug 27.
is a multidrug-resistant Gram-negative bacterium that poses a significant threat to public health, necessitating rapid and on-site detection methods for rapid recognition. The goal of the project is therefore to indirectly detect the presence of in environmental water samples targeting one of its quorum-sensing molecules, namely, ()--butyryl homoserine lactone (BHL). To this aim, molecularly imprinted polymers (MIPs) were synthesized via bulk free-radical polymerization using BHL as a template molecule. The obtained MIP particles were immobilized onto screen-printed electrodes (MIP-SPEs), and the BHL rebinding was analyzed via electrochemical impedance spectroscopy (EIS). To study the specificity of the synthesized MIPs, isotherm curves were built after on-point rebinding analysis performed via LC-MS measurements for both MIPs and NIPs (nonimprinted polymers, used as a negative control), obtaining an imprinting factor (IF) of 2.8 (at = 0.4 mM). The MIP-SPEs were integrated into an electrochemical biosensor with a linear range of 1 × 10-1 × 10 nM and a limit of detection (LoD) of 31.78 ± 4.08 nM. Selectivity measurements were also performed after choosing specific interferent molecules, such as structural analogs and potential interferents, followed by on-point analysis performed in spiked tap water to prove the sensor's potential to detect the presence of the quorum-sensing molecule in environmentally related real-life samples.
是一种耐多药革兰氏阴性菌，对公众健康构成重大威胁，因此需要快速现场检测方法以实现快速识别。该项目的目标是间接检测环境水样中是否存在，目标是其群体感应分子之一，即（）-丁酰高丝氨酸内酯（BHL）。为此，以BHL为模板分子，通过本体自由基聚合合成了分子印迹聚合物（MIP）。将获得的MIP颗粒固定在丝网印刷电极（MIP-SPE）上，并通过电化学阻抗谱（EIS）分析BHL的再结合。为了研究合成MIP的特异性，在通过LC-MS测量对MIP和NIP（非印迹聚合物，用作阴性对照）进行逐点再结合分析后绘制等温线，得到的印迹因子（IF）为2.8（在=0.4 mM时）。MIP-SPE被集成到一个电化学生物传感器中，线性范围为1×10-1×10 nM，检测限（LoD）为31.78±4.08 nM。在选择特定的干扰分子，如结构类似物和潜在干扰物后，也进行了选择性测量，然后在加标自来水中进行逐点分析，以证明该传感器在环境相关实际样品中检测群体感应分子存在的潜力。
