肉桂酸可减弱铜绿假单胞菌PAO1中群体感应相关的毒力因子及生物膜形成。
Cinnamic acid attenuates quorum sensing associated virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1.
作者信息
Rajkumari Jobina, Borkotoky Subhomoi, Murali Ayaluru, Suchiang Kitlangki, Mohanty Saswat Kumar, Busi Siddhardha
机构信息
Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry, 605014, India.
Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry, India.
出版信息
Biotechnol Lett. 2018 Jul;40(7):1087-1100. doi: 10.1007/s10529-018-2557-9. Epub 2018 Apr 21.
OBJECTIVE
Anti-quorum sensing and anti-biofilm efficacy of Cinnamic acid against Pseudomonas aeruginosa was comparatively assessed with respect to potent quorum sensing inhibitor, Baicalein.
RESULTS
At sub-lethal concentration, Cinnamic acid effectively inhibited both the production of the QS-dependent virulence factors and biofilm formation in P. aeruginosa without affecting the viability of the bacterium. The phytocompound interfered with the initial attachment of planktonic cells to the substratum thereby causing reduction in biofilm development. In addition, the in vivo study indicated that the test compound protected Caenorhabditis elegans from the virulence factors of P. aeruginosa leading to reduced mortality. The in silico analysis revealed that Cinnamic acid can act as a competitive inhibitor for the natural ligands towards the ligand binding domain of the transcriptional activators of the quorum sensing circuit in P. aeruginosa, LasR and RhlR.
CONCLUSIONS
The findings suggest that Cinnamic acid may serve as a novel quorum sensing based anti-infective in controlling P. aeruginosa infections.
目的
比较肉桂酸与强效群体感应抑制剂黄芩苷对铜绿假单胞菌的群体感应抑制和抗生物膜效果。
结果
在亚致死浓度下，肉桂酸有效抑制了铜绿假单胞菌中群体感应依赖性毒力因子的产生和生物膜形成，且不影响细菌的活力。该植物化合物干扰了浮游细胞与基质的初始附着，从而减少了生物膜的形成。此外，体内研究表明，受试化合物可保护秀丽隐杆线虫免受铜绿假单胞菌毒力因子的侵害，导致死亡率降低。计算机模拟分析表明，肉桂酸可作为天然配体对铜绿假单胞菌群体感应回路转录激活因子LasR和RhlR的配体结合域的竞争性抑制剂。
结论
研究结果表明，肉桂酸可能作为一种新型的基于群体感应的抗感染药物来控制铜绿假单胞菌感染。
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