Phyto-mediated synthesis of nanoparticles and their applications on hydrogen generation on NaBH, biological activities and photodegradation on azo dyes: Development of machine learning model.

This work reports the synthesis of the silver-platinum bimetallic nanoparticles (N@Pt-Ag BNPs) reduced by an ethanolic extract of black seed (Nigella sativa, N) using the green synthesis method, these nanoparticles show a great antibacterial, anticancer, and catalytic activity. The characterization of physicochemical properties of Ag-Pt BNP was carried out using UV-visible spectroscopy (Uv-vis), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and Transmission electron microscope (TEM) analysis. The structural morphology shows that the N@Pt-Ag BNPs are spherical particles with a diameter of 5.6 nm. The cytotoxic effects of N@Pt-Ag BNPs were examined by MTT test in human breast cancer, human colon cancer, human pancreatic cancer, L929-Murine fibroblast cells. N@Pt-Ag BNPs have been observed to be much more effective in breast cancer cell lines. The cytotoxic effect of N@Pt-Ag BNPs against healthy L929-murine fibroblast cell lines was not observed. Also, high antibacterial activity on each of the bacteria Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus (S. aureus), where we note that most strains of E. coli and S. aureus were damaged with a 73% percentage, 67% bacterial inhibition respectively. The results of the catalytic activities of N@Pt-Ag BNPs were obtained by performing the hydrolysis experiments of sodium borohydride (NaBH). According to the results obtained, TOF, enthalpy, entropy, and activation energy, values were found to be 2497.14 h, 13.52 kJ/mol, -137.47 J/mol.K, 16.02 kJ/mol, respectively. N@Pt-Ag BNPs were found to be highly effective catalysts for hydrogen production which this was also confirmed by the machine learning model. The photocatalytic activity of N@Pt-Ag BNPs was tested against methylene blue (MB) dye and the highest activity was found as 80%.