Department of Biotechnology, University of Science and Technology, Bannu 28100, Pakistan.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Molecules. 2022 Nov 15;27(22):7895. doi: 10.3390/molecules27227895.
Nanotechnology is one of the most recent technologies. It is uncertain whether the production of small-size nanoparticles (NPs) can be achieved through a simple, straightforward, and medicinally active phytochemical route. The present study aimed to develop an easy and justifiable method for the synthesis of Ag, Au, and their Ag/Au bimetallic NPs (BNPs) by using (HH) extract, and then to investigate the effects of Ag, Au, and their Ag/Au BNPs as antimicrobial and phytotoxic agents. Ag, Au, and their Ag/Au BNPs were characterized by UV-visible spectroscopy, FT-IR spectroscopy, XRD, EDX, and SEM analysis. XRD analysis conferring to the face of face-centered cubic crystal structure with an average size of 13.3, 10.72, and 8.34 nm of Ag, Au, and Ag/Au BNPs, respectively. SEM showed that Ag, Au, and Ag/Au BNPs had spherical morphologies, with calculated nano measurements of 40, 30, and 20 nm, respectively. The EDX analysis confirmed the composition of elemental Ag signal of the HH-AgNPs with 22.75%, Au signal of the HH-AuNPs with 48.08%, Ag signal with 12%, and Au signal with 38.26% of the Ag/Au BNPs. The Ag/Au BNPs showed an excellent antimicrobial efficacy against Gram-positive , , , , , , , and Gram-negative , , and bacterial strains, as well as against three fungal strains (, , and ) compared to HH extract, HH-AgNPs, and HH-AuNPs. However, further investigations are recommended to be able to minimize potential risks of application.
纳米技术是最新的技术之一。目前还不确定是否可以通过简单、直接和具有药用活性的植物化学途径来生产小尺寸的纳米颗粒 (NPs)。本研究旨在开发一种简单且合理的方法，使用 HH 提取物合成 Ag、Au 及其 Ag/Au 双金属 NPs（BNPs），然后研究 Ag、Au 及其 Ag/Au BNPs 作为抗菌和植物毒性剂的作用。Ag、Au 及其 Ag/Au BNPs 通过紫外-可见光谱、傅里叶变换红外光谱、X 射线衍射、能谱分析和扫描电子显微镜分析进行表征。X 射线衍射分析表明，Ag、Au 及其 Ag/Au BNPs 具有面心立方晶体结构，平均粒径分别为 13.3、10.72 和 8.34nm。SEM 显示 Ag、Au 和 Ag/Au BNPs 具有球形形态，计算出的纳米尺寸分别为 40、30 和 20nm。EDX 分析证实了 HH-AgNPs 中元素 Ag 信号的组成，Ag 信号的含量为 22.75%，HH-AuNPs 中 Au 信号的含量为 48.08%，Ag/Au BNPs 中 Ag 信号的含量为 12%，Au 信号的含量为 38.26%。与 HH 提取物、HH-AgNPs 和 HH-AuNPs 相比，Ag/Au BNPs 对革兰氏阳性菌 、 、 、 、 、 和革兰氏阴性菌 、 和 具有极好的抗菌功效，以及对三种真菌菌株（ 、 和 ）也具有极好的抗菌功效。然而，建议进行进一步的研究，以能够最小化应用的潜在风险。
