Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh 201301, India.
Diatom Research Laboratory, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh 201301, India.
Bioresour Technol. 2022 May;352:127128. doi: 10.1016/j.biortech.2022.127128. Epub 2022 Apr 7.
Diatoms are of great interest for many biotechnological applications. The present study highlights the comparative analysis for mass cultivation under the effect of seawater made from table salt (TS), rock salt (RS), and synthetic seawater in the presence of normal silica and induction coupled plasma (ICP) nanosilica (Nano Si) for inducing diatom growth. Out of all the test formulations, RS-f/2 Nano Si showed the best results with maximum cell density (3.16x107±0.04 and 3.24x107±0.05 cells mL), carbohydrate (403.0±3.4 and 398.0±8.1 mg g), and chrysolaminarin yield (66.2±5.5 and 49.3±5.1 mg g) in both Chaetoceros gracilis and Thalassiosira weissflogii respectively. The presence of a rich pigment profile and lipids further highlights the importance of TS and RS for cost-effective mass culturing. Results reveal that mass cultivation of marine diatoms with TS and RS in the presence of nanosilica not only reduces costs but also enhances metabolite production.
硅藻在许多生物技术应用中具有重要意义。本研究强调了在正常硅和感应耦合等离子体（ICP）纳米硅（Nano Si）存在下，从食盐（TS）、岩盐（RS）和合成海水对大量培养的比较分析，以诱导硅藻生长。在所有测试配方中，RS-f/2 Nano Si 表现最佳，其最大细胞密度（3.16x107±0.04 和 3.24x107±0.05 个细胞/mL）、碳水化合物（403.0±3.4 和 398.0±8.1 mg/g）和金藻昆布糖产量（66.2±5.5 和 49.3±5.1 mg/g）在葛氏角毛藻和威氏海链藻中均有表现。丰富的色素谱和脂质的存在进一步强调了 TS 和 RS 对经济高效的大规模培养的重要性。结果表明，在纳米硅存在下，用 TS 和 RS 进行海洋硅藻的大规模培养不仅降低了成本，而且提高了代谢产物的产量。
