Institute of Marine Research, P.O. Box 1870 Nordnes, NO-5817, Bergen, Norway; Dept. of Medical Biology, Faculty of Health Sciences, University of Tromsø-The Arctic University of Norway, 9037, Tromsø, Norway.
Dept. of Medical Microbiology, Stavanger University Hospital, 4011, Stavanger, Norway; Dept. of Biological Sciences, Faculty of Mathematics and Natural Sciences, University of Bergen, 5006, Bergen, Norway.
Int J Hyg Environ Health. 2022 May;242:113967. doi: 10.1016/j.ijheh.2022.113967. Epub 2022 Apr 7.
Klebsiella pneumoniae (Kp) can cause hospital- and community acquired infections. Although, Kp is widespread in the environment, very little is known about the genetic diversity and pathogenicity of Kp from the marine environment. The aim of our study was to understand the genetic diversity, resistome and pathogenic potential of 87 Kp isolates from the Norwegian marine environment, using whole-genome sequencing. We identified 50 sequence types, including globally disseminated sequence types associated with multidrug resistance or hypervirulence. Ten isolates carried the yersiniabactin loci. Acquired antibiotic resistance genes were identified in six Kp isolates. Heavy metal resistance genes were widespread among the isolates, with 71% carrying genes encoding resistance to copper, silver, arsenic, nickel and/or mercury. Co-occurrence of antibiotic resistance genes and heavy metal resistance genes was seen in five Kp isolates. Phylogenetic analysis revealed a close genetic relationship between Kp 2016-1200 ST25 isolated from blue mussels (Mytilus edulis) and a clinical isolate reported in Germany. To the best of our knowledge, this study provides the first comprehensive account of genetic diversity among Kp from the marine environment. Our study reveals high diversity of Kp in the Norwegian marine environment and seafood, including globally disseminated pathogenic sequence types carrying clinically relevant antibiotic resistance genes and virulence factors, as well as several heavy metal resistance genes.
肺炎克雷伯菌（Kp）可引起医院获得性和社区获得性感染。尽管 Kp 在环境中广泛存在，但对于海洋环境中 Kp 的遗传多样性和致病性知之甚少。我们的研究旨在通过全基因组测序了解来自挪威海洋环境的 87 株 Kp 的遗传多样性、耐药组和致病性。我们确定了 50 个序列类型，包括与多药耐药或高致病性相关的全球传播的序列类型。10 株分离株携带耶尔森菌素基因座。在 6 株 Kp 分离株中发现了获得性抗生素耐药基因。重金属耐药基因在分离株中广泛存在，71%的分离株携带编码对铜、银、砷、镍和/或汞耐药的基因。在 5 株 Kp 分离株中观察到抗生素耐药基因和重金属耐药基因的共存。系统发育分析显示，从贻贝（Mytilus edulis）中分离到的 Kp 2016-1200 ST25 与德国报道的临床分离株之间存在密切的遗传关系。据我们所知，这项研究首次全面描述了海洋环境中 Kp 的遗传多样性。我们的研究揭示了挪威海洋环境和海鲜中 Kp 的多样性很高，包括具有临床相关抗生素耐药基因和毒力因子的全球传播的致病性序列类型，以及几个重金属耐药基因。
