Fujimoto T, Singer S J
J Cell Biol. 1986 Dec;103(6 Pt 2):2775-86. doi: 10.1083/jcb.103.6.2775.
It is currently believed that the intermediate filaments of endothelial cells contain vimentin subunits exclusively. This inference, however, is derived from studies of only a few types of endothelial cells. By double indirect immunofluorescence and immunoelectron microscopy, we have now examined the endothelial cells of the micro- and macrovasculature of a variety of tissues and organs of adult chicken in vivo for their content of desmin and vimentin. Endothelial cells of the peritubular capillary in the renal cortex, the hepatic sinusoid, and the splenic sinusoid were found to contain only desmin; those of the exocrine pancreas capillary contained both desmin and vimentin; and the endothelial cells of the macrovasculatures and of all the other microvasculatures examined, including the vasa recta of the renal medulla, contained only vimentin. Such heterogeneity suggests that different types of adult chicken endothelial cells may have different embryological origins. To the extent that desmin and vimentin intermediate filaments may be functionally distinct, these results also suggest that different capillary endothelial cells may have different functional properties.
目前认为，内皮细胞的中间丝仅包含波形蛋白亚基。然而，这一推断仅来自对少数几种内皮细胞的研究。通过双重间接免疫荧光和免疫电子显微镜技术，我们现在对成年鸡体内各种组织和器官的微血管和大血管的内皮细胞进行了检测，以确定其结蛋白和波形蛋白的含量。发现肾皮质肾小管周围毛细血管、肝血窦和脾血窦的内皮细胞仅含有结蛋白；外分泌胰腺毛细血管的内皮细胞同时含有结蛋白和波形蛋白；而包括肾髓质直小血管在内的所有检测的大血管和其他微血管的内皮细胞仅含有波形蛋白。这种异质性表明，成年鸡不同类型的内皮细胞可能有不同的胚胎起源。就结蛋白和波形蛋白中间丝可能在功能上有所不同而言，这些结果还表明，不同的毛细血管内皮细胞可能具有不同的功能特性。
