Fujimoto T, Singer S J
J Histochem Cytochem. 1987 Oct;35(10):1105-15. doi: 10.1177/35.10.3305702.
The composition of intermediate filaments in pericytes was examined by immunofluorescent and immunoelectron microscopic labeling of frozen sections of various chicken microvascular beds in situ. Pericytes in capillaries of cardiac muscle, exocrine pancreas, and kidney (peritubular capillary) were found to contain both desmin and vimentin. In some capillaries where pericytes do not exist, cells apposed to endothelial cells--the Ito cell in the hepatic sinusoid and the reticular cell in the splenic sinusoid--were shown to contain both of the intermediate filament proteins. In contrast, podocytes and mesangial cells around renal glomerular capillaries contained only vimentin. The presence of desmin supports the hypothesis that pericytes may have a contractile apparatus similar to that of vascular smooth muscle cells. Our results also revealed that even in microvascular beds where pericytes are not found, cells having both desmin and vimentin exist next to endothelial cells and may assume similar functions to pericytes.
通过对各种鸡微血管床原位冷冻切片进行免疫荧光和免疫电子显微镜标记，研究了周细胞中间丝的组成。发现心肌、外分泌胰腺和肾脏（肾小管周围毛细血管）毛细血管中的周细胞同时含有结蛋白和波形蛋白。在一些不存在周细胞的毛细血管中，与内皮细胞相邻的细胞——肝血窦中的贮脂细胞和脾血窦中的网状细胞——被证明同时含有这两种中间丝蛋白。相比之下，肾小球毛细血管周围的足细胞和系膜细胞仅含有波形蛋白。结蛋白的存在支持了周细胞可能具有与血管平滑肌细胞类似的收缩装置这一假说。我们的结果还表明，即使在未发现周细胞的微血管床中，同时含有结蛋白和波形蛋白的细胞也存在于内皮细胞旁边，并且可能承担与周细胞类似的功能。
