四氢嗜热球菌新型胞外多糖的结构表征及生物活性。
Structural characterization and bioactivity of novel exopolysaccharides produced by Tetragenococcus halophilus.
机构信息
College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China; Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu 610065, China.
College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
出版信息
Food Res Int. 2022 May;155:111083. doi: 10.1016/j.foodres.2022.111083. Epub 2022 Mar 1.
In this study, exopolysaccharides produced by Tetragenococcus halophilus were extracted and purified. Two fractions named EPS-1 and EPS-2 were obtained with average molecular weights of 2613.4 and 93.4 kDa, respectively. Analysis of the structures demonstrated that EPS-1 was mainly composed of galactose, glucose, mannose and glucuronic acid, and EPS-2 mostly consisted of glucose and mannose. 800 MHz nuclear magnetic resonance spectroscopy elucidated different and partial primary structures of EPS-1 and EPS-2. The microtopography was observed by atomic force microscope and scanning electron microscope. In addition, the two fractions exhibited antioxidant activities on DPPH, hydroxyl and ABTS radicals. Furthermore, EPS-1 and EPS-2 could protect Lactococcus lactis against cryogenic and lyophilized stress. These results indicated that exopolysaccharides from T. halophilus in this study would have great potential in the application of food and pharmaceutical industry.
在这项研究中，从嗜热链球菌中提取并纯化了胞外多糖。得到了两个分别命名为 EPS-1 和 EPS-2 的组分，其平均分子量分别为 2613.4 和 93.4 kDa。结构分析表明，EPS-1 主要由半乳糖、葡萄糖、甘露糖和葡萄糖醛酸组成，而 EPS-2 主要由葡萄糖和甘露糖组成。800 MHz 核磁共振波谱阐明了 EPS-1 和 EPS-2 的不同和部分一级结构。原子力显微镜和扫描电子显微镜观察了其微观形貌。此外，这两个组分在 DPPH、羟基和 ABTS 自由基清除方面均表现出抗氧化活性。此外，EPS-1 和 EPS-2 可以保护乳球菌对抗低温和冻干应激。这些结果表明，本研究中来自嗜热链球菌的胞外多糖在食品和制药工业的应用中具有巨大潜力。
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