Liu Lina, Xu Jiaju, Na Ruiying, Du Renpeng, Ping Wenxiang, Ge Jingping, Zhao Dan
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, PR China.
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, PR China; Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080, PR China; Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008, PR China.
Int J Biol Macromol. 2022 May 1;206:777-787. doi: 10.1016/j.ijbiomac.2022.03.083. Epub 2022 Mar 18.
In this study, the exopolysaccharide (EPS) from Saccharomyces cerevisiae Y3 was extracted and purified, the preliminary structure characteristics and biological activities was investigated. The S. cerevisiae Y3 EPS was obtained by ethanol precipitation and gel filtration chromatography. M of purified Y3 EPS was 93,477 Da. High-performance liquid chromatography (HPLC), fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and methylation analyses showed that the EPS was a heteropolysaccharide, which composed of 1-α-D mannose (39.8%), 1,2-α-D mannose (19.6%), 1,6-α-D linked mannose (10.4%), 1,3,6-β-D glucose (27.5%), and 1-β-D linked glucose (1.9%). Scanning electron microscope (SEM) and atomic force microscope (AFM) further revealed smooth and dense sheet-like structure with reticular configuration. The Congo red test and X-ray diffraction (XRD) reflected an irregular coil conformation and non-crystalline amorphous nature. The EPS exhibited good hydrophilicity, thermal stability and antioxidation ability for DPPH radicals, hydroxyl radicals and NO, as well as good prebiotic properties. These results indicated that Y3 EPS could be explored as a promising functional adjunct for application in probiotics and antioxidation.
本研究对酿酒酵母Y3胞外多糖（EPS）进行了提取纯化，并对其初步结构特征和生物活性进行了研究。通过乙醇沉淀和凝胶过滤色谱法获得了酿酒酵母Y3 EPS。纯化后的Y3 EPS分子量为93,477 Da。高效液相色谱（HPLC）、傅里叶变换红外光谱（FT-IR）、核磁共振光谱（NMR）和甲基化分析表明，该EPS是一种杂多糖，由1-α-D-甘露糖（39.8%）、1,2-α-D-甘露糖（19.6%）、1,6-α-D-连接甘露糖（10.4%）、1,3,6-β-D-葡萄糖（27.5%）和1-β-D-连接葡萄糖（1.9%）组成。扫描电子显微镜（SEM）和原子力显微镜（AFM）进一步揭示了其具有光滑致密的片状结构和网状构型。刚果红试验和X射线衍射（XRD）反映出其具有不规则卷曲构象和非晶态无定形性质。该EPS对DPPH自由基、羟基自由基和NO表现出良好的亲水性、热稳定性和抗氧化能力，以及良好的益生元特性。这些结果表明，Y3 EPS有望作为一种有前途的功能性添加剂应用于益生菌和抗氧化领域。
