人类抗白色念珠菌抗体的独特型：重现性、交叉反应性自身抗独特型样活性的存在及其在体外特异性抗体应答诱导中的作用
Idiotypy of human anti-Candida albicans antibodies: recurrence, presence of a cross-reactive autoanti-idiotypic-like activity, and role in the induction of specific in vitro antibody response.
作者信息
de Saint Basile G, Durandy A, Somme G, Griscelli C
出版信息
J Immunol. 1987 Jan 15;138(2):417-22.
Rabbit anti-idiotypic antibodies (L12) were raised against human anti-mannan of Candida albicans (CA) antibodies isolated from the serum of a normal donor. The absorbed anti-idiotypic antiserum bound to donor anti-CA mannan antibodies but not to control immunoglobulins. Binding was inhibited by CA mannan but not by other polysaccharide antigens. L12 was shown to cross-react with anti-CA mannan-isolated antibodies or with anti-CA antibody-containing sera from individuals unrelated to the donor. IgG fraction isolated from the donor serum was repeatedly absorbed on CA mannan Sepharose to remove anti-mannan antibodies. This IgG fraction (named autoanti-idiotypic fraction) blocked, in a dose-dependent fashion, the binding of rabbit anti-idiotype to donor anti-CA mannan antibodies. Moreover, this CP-depleted IgG fraction cross-reacted with public idiotypic determinants of unrelated anti-CA mannan antibodies. Finally, L12 induced sensitized lymphocytes to produce anti-CA mannan antibodies in vitro in the absence of antigen.
兔抗独特型抗体（L12）是针对从一名正常供体血清中分离出的人抗白色念珠菌（CA）甘露聚糖抗体产生的。吸收后的抗独特型抗血清能与供体抗CA甘露聚糖抗体结合，但不与对照免疫球蛋白结合。CA甘露聚糖可抑制这种结合，而其他多糖抗原则无此作用。结果表明，L12与抗CA甘露聚糖分离抗体或与来自与供体无关个体的含抗CA抗体血清发生交叉反应。从供体血清中分离出的IgG组分在CA甘露聚糖琼脂糖上反复吸收以去除抗甘露聚糖抗体。该IgG组分（称为自身抗独特型组分）以剂量依赖方式阻断兔抗独特型与供体抗CA甘露聚糖抗体的结合。此外，这种去除补体的IgG组分与无关抗CA甘露聚糖抗体的公共独特型决定簇发生交叉反应。最后，在无抗原的情况下，L12在体外诱导致敏淋巴细胞产生抗CA甘露聚糖抗体。
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