黑麦敏感型过敏患者血清中与公共独特型决定簇发生交叉反应的自身抗独特型抗体的证明。

Demonstration of auto-anti-idiotypic antibody cross-reacting with public idiotypic determinants in the serum of rye-sensitive allergic patients.

作者信息

Bose R, Marsh D G, Duchateau J, Sehon A H, Delespesse G

出版信息

J Immunol. 1984 Nov;133(5):2474-8.

Abstract

The present study documents the presence, in the serum of one allergic individual, of auto-anti-idiotypic antibodies cross-reacting with public idiotypic determinants expressed on human IgE and IgG anti-Rye I antibodies. Sera from rye-sensitive patients were tested for specific IgG and IgE antibodies to Rye I by double antibody. The IgG fraction, isolated from the serum of a patient with a history of previous hyposensitization therapy, was repeatedly absorbed on Rye-I-Sepharose as well as on IgM- and IgG-Sepharose to remove anti-Rye I antibodies as well as any possible anti-heavy or light chain activity. This IgG fraction, named anti-idiotypic fraction (a-IdF), blocked in a dose-dependent fashion the reaction of IgG and IgE anti-Rye I antibodies with Rye I antigen. The a-IdF also blocked the binding of anti-rye antibodies to Rye I antigen in the serum of 20 unrelated allergic patients, indicating that these anti-Rye I antibodies bore public idiotypic determinants.

摘要

本研究证明，在一名过敏个体的血清中存在自身抗独特型抗体，该抗体与人类IgE和IgG抗黑麦I抗体上表达的公共独特型决定簇发生交叉反应。通过双抗体法检测黑麦敏感患者血清中针对黑麦I的特异性IgG和IgE抗体。从一名有过减敏治疗史患者的血清中分离出的IgG组分，在黑麦I-琼脂糖凝胶以及IgM和IgG-琼脂糖凝胶上反复吸附，以去除抗黑麦I抗体以及任何可能的抗重链或轻链活性。该IgG组分称为抗独特型组分（a-IdF），它以剂量依赖方式阻断IgG和IgE抗黑麦I抗体与黑麦I抗原的反应。a-IdF还阻断了20名无亲缘关系的过敏患者血清中抗黑麦抗体与黑麦I抗原的结合，表明这些抗黑麦I抗体带有公共独特型决定簇。