Geha R S
J Immunol. 1984 Oct;133(4):1846-51.
The role of idiotypic anti-idiotypic interactions in the regulation of the human T cell response to tetanus toxoid (TT) antigen was examined in three subjects. Rabbit anti-idiotypic (anti-Id) antisera were raised against IgG (Fab')2 anti-TT obtained 7 to 10 days after booster immunization with TT. F(ab')2 fragments of rabbit-anti-Id IgG were used in conjunction with fluorescein-conjugated goat anti-rabbit Ig in an indirect immunofluorescence assay to determine the frequency of Id-positive cells in T cell-enriched preparations. This frequency was 24, 29, and 38 per 10,000, respectively, in the three subjects studied. Significant contribution of contaminating B cell to fluorescence-staining was ruled out by capping experiments using goat anti-human Ig (GAHIG) and by double staining experiments using rhodamine-conjugated GAHIG. Absorption of anti-Id antisera with Epstein Barr virus (EBV)-transformed B cell lines from the IgG (Fab')2 anti-TT donor, but not with EBV-B cell lines from unrelated donors, removed their reactivity with the T cells. Rabbit anti-Id IgG caused minimal proliferation (two-threefold) of T cells and had no effect on T cell proliferation in response to TT antigen when added to the cultures. Preincubation of T cells for 48 hr with rabbit anti-Id IgG (Fab')2, but not with preimmune rabbit IgG (Fab')2, resulted in the generation of antigen-specific suppressor cells that inhibited T cell proliferation in response to TT, but not in response to diphtheria toxoid (DT). These cells also inhibited the synthesis of IgG anti-TT in response to in vitro stimulation with TT antigen, but not the synthesis of IgG anti-DT in response to DT antigen. Adsorption of T cells over plates coated with rabbit anti-Id IgG (Fab')2 enhanced the proliferative response of the T cells to TT, but not to DT antigen, and enhanced the helper activity of the T cells for the in vitro synthesis of IgG anti-TT but not of IgG anti-DT antibodies. These results suggest that idiotypic-anti-idiotypic interactions play a role in the human T cell response to antigen.
在三名受试者中研究了独特型-抗独特型相互作用在人T细胞对破伤风类毒素（TT）抗原反应调节中的作用。用TT加强免疫7至10天后获得的IgG（Fab'）2抗-TT制备兔抗独特型（抗-Id）抗血清。兔抗-Id IgG的F（ab'）2片段与异硫氰酸荧光素偶联的山羊抗兔Ig一起用于间接免疫荧光测定，以确定富含T细胞的制剂中Id阳性细胞的频率。在研究的三名受试者中，该频率分别为每10,000个细胞中有24、29和38个。使用山羊抗人Ig（GAHIG）的封帽实验以及使用罗丹明偶联的GAHIG的双重染色实验排除了污染B细胞对荧光染色的显著贡献。用来自IgG（Fab'）2抗-TT供体的爱泼斯坦-巴尔病毒（EBV）转化的B细胞系吸收抗-Id抗血清，但不与来自无关供体的EBV-B细胞系吸收，消除了它们与T细胞的反应性。兔抗-Id IgG引起T细胞的最小增殖（两到三倍），并且当添加到培养物中时，对T细胞对TT抗原的增殖没有影响。用兔抗-Id IgG（Fab'）2预孵育T细胞48小时，但不用免疫前兔IgG（Fab'）2预孵育，导致产生抗原特异性抑制细胞，其抑制T细胞对TT的增殖，但不抑制对白喉类毒素（DT）的增殖。这些细胞还抑制了在TT抗原体外刺激下IgG抗-TT的合成，但不抑制在DT抗原刺激下IgG抗-DT的合成。用涂有兔抗-Id IgG（Fab'）2的平板吸附T细胞增强了T细胞对TT的增殖反应，但不增强对DT抗原的增殖反应，并增强了T细胞对体外合成IgG抗-TT的辅助活性，但不增强对IgG抗-DT抗体的辅助活性。这些结果表明独特型-抗独特型相互作用在人T细胞对抗原的反应中起作用。
