Zeng Qi, Hu Beijuan, Blanco Andres Hortas, Zhang Wanchang, Zhao Daxian, Martínez Paulino, Hong Yijiang
School of Life Sciences, Nanchang University, Nanchang, China.
Key Lab of Aquatic Resources and Utilization of Jiangxi, Nanchang, China.
Front Genet. 2022 Mar 24;13:868742. doi: 10.3389/fgene.2022.868742. eCollection 2022.
The freshwater mussel is a cultured bivalve in China, and the quality of the pearls produced is affected by the type of gonads. However, because of the lack of a published genome and the complexity of sex determination, research on sex reversal and development of this species is limited. In this study, Illumina RNA-seq and PacBio Isoform Sequencing (Iso-Seq) were combined to analyze the gonads of . A total of 201,481 high-quality transcripts were generated. The study identified 7,922 differentially expressed genes in three comparison group (females versus males, hermaphrodites versus females, and hermaphrodites versus males). Twenty-four genes were identified as potential sex-related genes, including and involved in sex determination, and , and involved in gonadal development. We also speculated a possible pathways for the formation of hermaphroditism in . The data provide a clear view of the transcriptome for gonads and will be valuable in elucidating the mechanisms of gonad development.
淡水贻贝是中国一种养殖的双壳类动物，所产珍珠的质量受性腺类型的影响。然而，由于缺乏已发表的基因组以及性别决定的复杂性，该物种的性逆转和发育研究受到限制。在本研究中，结合了Illumina RNA测序和PacBio全长转录组测序（Iso-Seq）来分析[物种名称未给出]的性腺。共产生了201,481个高质量转录本。该研究在三个比较组（雌性与雄性、雌雄同体与雌性、雌雄同体与雄性）中鉴定出7,922个差异表达基因。确定了24个基因作为潜在的性别相关基因，包括参与性别决定的[基因名称未给出]和[基因名称未给出]，以及参与性腺发育的[基因名称未给出]、[基因名称未给出]和[基因名称未给出]。我们还推测了[物种名称未给出]中雌雄同体形成的可能途径。这些数据为[物种名称未给出]性腺的转录组提供了清晰的视图，将有助于阐明性腺发育的机制。
