Shi Jianwu, Hong Yijiang, Sheng Junqing, Peng Kou, Wang Junhua
a School of Life Sciences , Nanchang University , Nanchang , China.
Biosci Biotechnol Biochem. 2015;79(8):1257-65. doi: 10.1080/09168451.2015.1025690. Epub 2015 Apr 7.
This study presents the first analysis of expressed transcripts in the spermary and ovary of Hyriopsis schlegelii (H. schlegelii). A total of 132,055 unigenes were obtained and 31,781 of these genes were annotated. In addition, 19,511 upregulated and 25,911 downregulated unigenes were identified in the spermary. Ten sex-determination genes were selected and further analyzed by real-time PCR. In addition, mammalian genes reported to govern sex-determination pathways, including Sry, Dmrt1, Dmrt2, Sox9, GATA4, and WT1 in males and Wnt4, Rspo1, Foxl2, and β-catenin in females, were also identified in H. schlegelii. These results suggest that H. schlegelii and mammals use similar gene regulatory mechanisms to control sex determination. Moreover, genes associated with dosage compensation mechanisms, such as Msl1, Msl2, and Msl3, and hermaphrodite phenotypes, such as Tra-1, Tra-2α, Tra-2β, Fem1A, Fem1B, and Fem1C, were also identified in H. schlegelii. The identification of these genes indicates that diverse regulatory mechanisms regulate sexual polymorphism in H. schlegelii.
本研究首次对三角帆蚌精巢和卵巢中的表达转录本进行了分析。共获得132,055个单基因，其中31,781个基因得到注释。此外，在精巢中鉴定出19,511个上调单基因和25,911个下调单基因。选择了10个性别决定基因并通过实时PCR进一步分析。此外，在三角帆蚌中还鉴定出了据报道在哺乳动物中控制性别决定途径的基因，包括雄性中的Sry、Dmrt1、Dmrt2、Sox9、GATA4和WT1，以及雌性中的Wnt4、Rspo1、Foxl2和β-连环蛋白。这些结果表明，三角帆蚌和哺乳动物使用相似的基因调控机制来控制性别决定。此外，在三角帆蚌中还鉴定出了与剂量补偿机制相关的基因，如Msl1、Msl2和Msl3，以及与雌雄同体表型相关的基因，如Tra-1、Tra-2α、Tra-2β、Fem1A、Fem1B和Fem1C。这些基因的鉴定表明，多种调控机制调节三角帆蚌的性多态性。
