Liu Xu-Sheng, Kui Xue-Yan, Gao Yan, Chen Xue-Qin, Zeng Jing, Liu Xiao-Yu, Zhang Yu, Zhang Yao-Hua, Pei Zhi-Jun
Department of Nuclear Medicine and Institute of Anesthesiology and Pain, Taihe Hospital, Hubei University of Medicine, Shiyan, China.
Postgraduate Training Basement of Jinzhou Medical University, Taihe Hospital, Hubei University of Medicine, Shiyan, China.
Front Genet. 2022 Mar 23;13:835265. doi: 10.3389/fgene.2022.835265. eCollection 2022.
YTHDF1 is highly expressed in multiple tumors and affects tumor progression. However, there are only a few comprehensive studies on the analysis of YTHDF1 in esophageal cancer. We analyzed YTHDF1 expression in pan-cancer by comparing both the GEPIA and TCGA cohorts, and further verified the differences in YTHDF1 expression between the ESCA and normal groups by the GEO ESCA cohort and experiments. The correlation of YTHDF1 expression and the clinical characteristics of ESCA patients was analyzed using the TCGA ESCA clinical data. The GO and KEGG enrichment analyses of the YTHDF1 coexpressed genes were completed by bioinformatics analysis, and the GGI and PPI were constructed for the YTHDF1, respectively. The relationship between YTHDF1 expression and the infiltration of ESCA immune cells was analyzed by using the TIMER database and the TCGA ESCA cohort. The relationships between YTHDF1 expression levels and glycolysis and ferroptosis-related genes were analyzed using the TCGA and GEPIA ESCA cohorts. Finally, the ceRNA network that may be involved in YTHDF1 in ESCA was predicted and constructed through a variety of databases. YTHDF1 was overexpressed in various cancers, and experiments confirmed that YTHDF1 expression was higher in ESCA samples than in normal samples. The expression of YTHDF1 has some accuracy in predicting the tumor outcome. Expression of YTHDF1 was significantly associated with multiple clinical features in ESCA patients. GO and KEGG enrichment analyses indicated that YTHDF1 coexpressed genes involved multiple biological functions. There is a potential association between YTHDF1 expression and multiple immune cell infiltration, glycolysis, and ferroptosis-related genes in ESCA. YTHDF1 may be involved in multiple ceRNA regulatory networks in ESCA, including PAXIP1-AS1/hsa-miR-376c-3p/YTHDF1 axis, THUMPD3-AS1/hsa-miR-655-3p/YTHDF1 axis, and SNHG20/hsa-miR-655-3p/YTHDF1 axis, respectively. YTHDF1 can serve as a biomarker of ESCA, related to the immune cell infiltration of ESCA, regulation of glycolysis and ferroptosis, and the ceRNA regulatory network.
YTHDF1在多种肿瘤中高表达并影响肿瘤进展。然而，关于食管癌中YTHDF1的分析仅有少数全面的研究。我们通过比较GEPIA和TCGA队列分析了泛癌中YTHDF1的表达，并通过GEO食管癌队列和实验进一步验证了食管癌组与正常组之间YTHDF1表达的差异。使用TCGA食管癌临床数据分析YTHDF1表达与食管癌患者临床特征的相关性。通过生物信息学分析完成YTHDF1共表达基因的GO和KEGG富集分析，并分别构建YTHDF1的GGI和PPI。使用TIMER数据库和TCGA食管癌队列分析YTHDF1表达与食管癌免疫细胞浸润的关系。使用TCGA和GEPIA食管癌队列分析YTHDF1表达水平与糖酵解和铁死亡相关基因的关系。最后，通过多种数据库预测并构建了食管癌中可能涉及YTHDF1的ceRNA网络。YTHDF1在各种癌症中均过表达，实验证实食管癌样本中YTHDF1的表达高于正常样本。YTHDF1的表达在预测肿瘤结局方面具有一定准确性。YTHDF1的表达与食管癌患者的多种临床特征显著相关。GO和KEGG富集分析表明YTHDF1共表达基因涉及多种生物学功能。食管癌中YTHDF1表达与多种免疫细胞浸润、糖酵解和铁死亡相关基因之间存在潜在关联。YTHDF1可能参与食管癌中的多个ceRNA调控网络，分别包括PAXIP1-AS1/hsa-miR-376c- 3p/YTHDF1轴、THUMPD3-AS1/hsa-miR-655-3p/YTHDF1轴和SNHG20/hsa-miR-655-3p/YTHDF1轴。YTHDF1可作为食管癌的生物标志物，与食管癌的免疫细胞浸润、糖酵解和铁死亡调节以及ceRNA调控网络相关。
