Anderson J M, Soll D R
J Gen Microbiol. 1986 Jul;132(7):2035-47. doi: 10.1099/00221287-132-7-2035.
Stationary phase cells of Candida albicans can form either a bud or a hypha, depending upon the pH of the medium into which they are released. At low pH, cells form an ellipsoidal bud and at high pH, cells form an elongated hypha. By staining cells with rhodamine-conjugated phalloidin, we have compared the dynamics of actin localization during the formation of buds and hyphae. Before evagination, actin granules were distributed throughout the cytoplasmic cortex in both budding and hypha-forming cells. Just before evagination, actin granules clustered at the site of evagination, then filled the early evagination in both budding and hypha-forming cells. With continued bud growth, the actin granules then redistributed throughout the cytoplasmic cortex. In marked contrast, with continued hyphal growth, the majority of actin granules clustered at the hyphal apex. This distinct difference in actin granule localization may be related to the distinct differences in the expansion zones of the cell wall recently demonstrated between growing buds and hyphae. The spatial and temporal dynamics of the large neck actin granules and of actin fibres are also described.
白色念珠菌的静止期细胞能够形成芽体或菌丝，这取决于它们所处培养基的pH值。在低pH值条件下，细胞形成椭圆形芽体；在高pH值条件下，细胞形成细长的菌丝。通过用罗丹明标记的鬼笔环肽对细胞进行染色，我们比较了芽体和菌丝形成过程中肌动蛋白定位的动态变化。在出芽前，肌动蛋白颗粒分布在出芽细胞和形成菌丝的细胞的整个细胞质皮质中。就在出芽前，肌动蛋白颗粒聚集在出芽部位，然后填充到出芽细胞和形成菌丝的细胞的早期芽体中。随着芽体的持续生长，肌动蛋白颗粒随后重新分布在整个细胞质皮质中。与之形成显著对比的是，随着菌丝的持续生长，大多数肌动蛋白颗粒聚集在菌丝顶端。肌动蛋白颗粒定位的这种明显差异可能与最近在生长的芽体和菌丝之间所显示的细胞壁扩展区域的明显差异有关。还描述了大颈部肌动蛋白颗粒和肌动蛋白纤维的时空动态变化。
