Soll D R, Mitchell L H
J Cell Biol. 1983 Feb;96(2):486-93. doi: 10.1083/jcb.96.2.486.
Stationary phase cultures of Candida albicans inoculated into fresh medium at 37 degrees C synchronously from buds at pH 4.5 and mycelia at pH 6.5. During bud formation, a filament ring forms just under the plasma membrane at the mother cell-bud junction at roughly the time of evagination. A filament ring also forms in mycelium-forming cells, but it appears later than in a budding cell and it is positioned along the elongating mycelium, on the average 2 microns from the mother cell-mycelium junction. Sections of filament rings in early and late budding cells and in mycelia appear similar. Each contains approximately 11 to 12 filaments equidistant from one another and closely associated with the plasma membrane. In both budding and mycelium-forming cells, the filament ring disappears when the primary septum grows inward. The close temporal and spatial association of the filament ring and the subsequent chitin-containing septum suggests a role for the filament ring in septum formation. In addition, a close temporal correlation is demonstrated between filament ring formation and the time at which cells become committed to bud formation at pH 4.5 and mycelium formation at pH 6.5. The temporal and spatial differences in filament ring formation between the two growth forms also suggest a simple model for the positioning of the filament ring.
将白色念珠菌的稳定期培养物在37摄氏度下接种到新鲜培养基中，在pH 4.5时从芽同步生长，在pH 6.5时从菌丝体同步生长。在芽形成过程中，大约在出芽时，在母细胞 - 芽交界处的质膜下方会形成一个丝状环。在形成菌丝体的细胞中也会形成丝状环，但它比在芽殖细胞中出现得晚，并且沿着伸长的菌丝体定位，平均距离母细胞 - 菌丝体交界处2微米。早期和晚期芽殖细胞以及菌丝体中丝状环的切片看起来相似。每个丝状环包含大约11到12根彼此等距且与质膜紧密相关的细丝。在芽殖细胞和形成菌丝体的细胞中，当初级隔膜向内生长时，丝状环都会消失。丝状环与随后含几丁质的隔膜在时间和空间上的紧密关联表明丝状环在隔膜形成中起作用。此外，在pH 4.5时细胞开始芽殖以及在pH 6.5时细胞开始形成菌丝体的时间与丝状环形成之间存在紧密的时间相关性。两种生长形式之间丝状环形成的时间和空间差异也提示了一个关于丝状环定位的简单模型。
