Zhu Junwei, Ren Yunxiao, Han Yuanyuan, Jin Tingting, Li Yanming, Ruan Xiuyan, Qu Hongzhu, Huang Shengwen, Zhang Zhaojun, Fang Xiangdong
CAS Key Laboratory of Genome Science & Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.
Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China.
Blood Sci. 2019 Oct 21;1(2):161-167. doi: 10.1097/BS9.0000000000000031. eCollection 2019 Oct.
Erythropoiesis is a complex and sophisticated multi-stage process regulated by a variety of factors, including the transcription factor GATA1 and non-coding RNA. GATA1 is regarded as an essential transcriptional regulator promoting transcription of erythroid-specific genes-such as long non-coding RNAs (lncRNA). Here, we comprehensively screened lncRNAs that were potentially regulated by GATA1 in erythroid cells. We identified a novel lncRNA--and verified its role in promoting erythroid differentiation of K562 erythroid cells. We also predicted a model in which participates in erythroid differentiation via dynamic chromatin remodeling involving GATA1. The relationship between lncRNA and chromatin in the process of erythroid differentiation remains to be revealed, and in our study we have carried out preliminary explorations.
红细胞生成是一个复杂且精细的多阶段过程，受多种因素调控，包括转录因子GATA1和非编码RNA。GATA1被视为促进红系特异性基因（如长链非编码RNA，lncRNA）转录的关键转录调节因子。在此，我们全面筛选了红系细胞中可能受GATA1调控的lncRNA。我们鉴定出一种新型lncRNA，并验证了其在促进K562红系细胞红系分化中的作用。我们还预测了一个模型，其中lncRNA通过涉及GATA1的动态染色质重塑参与红系分化。lncRNA与染色质在红系分化过程中的关系仍有待揭示，在我们的研究中已进行了初步探索。
