Zhouravleva G A, Bondarev S A, Zemlyanko O M, Moskalenko S E
Department of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, 199034 Russia.
Laboratory of Amyloid Biology, St. Petersburg State University, St. Petersburg, 199034 Russia.
Mol Biol (Mosk). 2022 Mar-Apr;56(2):206-226. doi: 10.31857/S002689842201013X.
The review discusses the role that proteins interacting with the translation termination factors eRF1 and eRF3 play in the control of protein synthesis and prionization. These proteins interact not only with each other, but also with many other proteins involved in controlling the efficiency of translation termination, and associate translation termination with other cell processes. The termination of translation is directly related not only to translation re-initiation and ribosome recycling, but also to mRNA stability and protein quality control. This connection is ensured by the interaction of eRF1 and eRF3 with proteins participating in various cell metabolic processes, such as mRNA transport from the nucleus into the cytoplasm (Dbp5/DDX19 and Gle1), ribosome recycling (Rli1/ABCE1), mRNA degradation (Upf proteins), and translation initiation (Pab1/PABP). In addition to genetic control, there is epigenetic control of translation termination. This mechanism is associated with prion polymerization of the Sup35 protein to form the [PSI^(+)] prion. The maintenance of the [PSI^(+)] prion, like other yeast prions, requires the operation of a system of molecular chaperones and protein sorting factors. The review considers in detail the interaction of the translation termination factors with proteins involved in various cellular processes.
这篇综述讨论了与翻译终止因子eRF1和eRF3相互作用的蛋白质在蛋白质合成控制和朊病毒化过程中所起的作用。这些蛋白质不仅相互作用，还与许多其他参与控制翻译终止效率的蛋白质相互作用，并将翻译终止与其他细胞过程联系起来。翻译终止不仅与翻译重新起始和核糖体循环直接相关，还与mRNA稳定性和蛋白质质量控制有关。eRF1和eRF3与参与各种细胞代谢过程的蛋白质相互作用，确保了这种联系，这些过程包括mRNA从细胞核转运到细胞质（Dbp5/DDX19和Gle1）、核糖体循环（Rli1/ABCE1）、mRNA降解（Upf蛋白）以及翻译起始（Pab1/PABP）。除了遗传控制外，翻译终止还存在表观遗传控制。这种机制与Sup35蛋白的朊病毒聚合形成[PSI^(+)]朊病毒有关。与其他酵母朊病毒一样，[PSI^(+)]朊病毒的维持需要分子伴侣系统和蛋白质分选因子的运作。这篇综述详细探讨了翻译终止因子与参与各种细胞过程的蛋白质之间的相互作用。
