College of Ocean Food and Biological Engineering, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, 361021, China.
University of Texas at Austin, Austin, TX 78712, USA.
J Mater Chem B. 2022 Sep 15;10(35):6752-6757. doi: 10.1039/d2tb00263a.
β-Lactoglobulin (β-Lg), a food allergen, can easily cause allergic reactions in infants and young children. Therefore, it is necessary to develop a rapid, sensitive, and selective detection method to protect individuals prone to allergies. In this paper, a fluorescence assay based on WS nanosheets and a fluorescent dye (FAM)-labeled β-Lg aptamer was designed to detect β-Lg rapidly with high sensitivity. In the sensing platform, the β-Lg aptamer is adsorbed on the WS nanosheet surface by van der Waals forces, which trigger the phenomenon of fluorescence resonance energy transfer (FRET) and suppress the fluorescence signal in the system. When β-Lg is present, the conformation of the aptamer specifically bound to β-Lg changes. Therefore, the aptamer is separated from the WS nanosheet surface, and the fluorescence signal is recovered. This method combines the high quenching efficiency of WS nanosheets and good specificity of the β-Lg aptamer. The detection range of this method for β-Lg is 0.1-100 μg mL. The detection limit is 20.4 ng mL. This method exhibits high sensitivity, selectivity and good reproducibility, and it can be used for β-Lg detection in actual samples.
β-乳球蛋白（β-Lg）是一种食物过敏原，容易引起婴幼儿过敏反应。因此，有必要开发一种快速、灵敏、选择性的检测方法来保护易过敏个体。本文设计了一种基于 WS 纳米片和荧光染料（FAM）标记的β-Lg 适体的荧光测定法，用于快速高灵敏度检测β-Lg。在传感平台中，β-Lg 适体通过范德华力吸附在 WS 纳米片表面，触发荧光共振能量转移（FRET）现象，抑制体系中的荧光信号。当存在β-Lg 时，特异性结合β-Lg 的适体构象发生变化。因此，适体从 WS 纳米片表面分离，荧光信号得到恢复。该方法结合了 WS 纳米片的高猝灭效率和β-Lg 适体的良好特异性。该方法检测β-Lg 的范围为 0.1-100 μg mL，检测限为 20.4 ng mL。该方法具有高灵敏度、选择性和良好的重现性，可用于实际样品中的β-Lg 检测。
