Department of Ophthalmology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
Pharmaceutics and Pharmacology Department, Santen Pharmaceutical Co., Ltd., Nara, Japan.
Transl Vis Sci Technol. 2022 Apr 1;11(4):9. doi: 10.1167/tvst.11.4.9.
To evaluate tissue reactivity to, and the stability of, glaucoma drainage device materials placed under rabbit conjunctiva in vivo.
Disks (diameter, 3 mm; thickness, ∼0.3 mm) fabricated from poly(styrene-block-isobutylene-block-styrene) (SIBS), silicone, stainless-steel, or glutaraldehyde cross-linked collagen (GACLC) were inserted under rabbit conjunctiva. Conjunctival and scleral sections obtained at 4, 8, and 12 weeks after surgery were immunostained for α-smooth muscle actin (SMA). The ratio of the maximum thickness of the α-SMA-positive conjunctiva to the scleral thickness (α-SMA/S ratio) was calculated. The in vivo stability of the drainage devices at 12 weeks after insertion was evaluated.
The mean α-SMA/S ratios of the SIBS and silicone groups were lower than that of the stainless-steel group at 4 weeks after surgery (P < 0.05), and that of the SIBS group was lower than that of the GACLC group (P < 0.05). The ratios at 8 weeks after surgery were lower in the SIBS and silicone groups than in the GACLC group (P < 0.01). The ratios at 12 weeks after surgery were lower in the SIBS and silicone groups than in the GACLC group (P < 0.05). The surface areas of GACLC disks explanted from conjunctivae were significantly lower than that of intact disks (P < 0.01).
SIBS and silicon were highly biostable and exhibited less tissue reactivity than GACLC in vivo.
Comparisons of materials using animal models can predict the clinical stability and safety of such materials in humans.
评估体内兔结膜下青光眼引流装置材料的组织反应性和稳定性。
将直径为 3mm、厚度约为 0.3mm 的聚（苯乙烯-嵌段-异丁烯-嵌段-苯乙烯）（SIBS）、硅酮、不锈钢或戊二醛交联胶原（GACLC）制成的圆盘插入兔结膜下。术后 4、8 和 12 周获取结膜和巩膜切片，免疫染色α-平滑肌肌动蛋白（SMA）。计算 SMA 阳性结膜的最大厚度与巩膜厚度的比值（α-SMA/S 比值）。评估植入后 12 周引流装置的体内稳定性。
术后 4 周时，SIBS 和硅酮组的平均α-SMA/S 比值低于不锈钢组（P<0.05），SIBS 组低于 GACLC 组（P<0.05）。术后 8 周时，SIBS 和硅酮组的比值低于 GACLC 组（P<0.01）。术后 12 周时，SIBS 和硅酮组的比值低于 GACLC 组（P<0.05）。从结膜下取出的 GACLC 圆盘的表面积明显低于完整圆盘（P<0.01）。
SIBS 和硅酮在体内具有高度的生物稳定性，组织反应性低于 GACLC。
马冬梅
