评估玻璃体半乳甘露聚糖和（1,3）β-D-葡聚糖水平在印度南部真菌性眼内炎诊断中的应用。

Evaluation of Vitreous Galactomannan and (1, 3) β-D-Glucan Levels in the Diagnosis of Fungal Endophthalmitis in Southern India.

机构信息

Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L V Prasad Eye Institute, Hyderabad, India.

Centre for Doctoral Studies, Manipal Academy of Higher Education, Manipal, India.

出版信息

Ocul Immunol Inflamm. 2023 May;31(4):734-740. doi: 10.1080/09273948.2022.2060261. Epub 2022 Apr 11.

DOI:10.1080/09273948.2022.2060261

PMID:35404755

Abstract

PURPOSE

To evaluate vitreous Galactomannan(GM) and 1,3 β-D-Glucan (BDG) levels in the diagnosis of fungal endophthalmitis, with emphasis on culture-negative cases.

METHODS

Vitreous from 31 clinically suspected fungal endophthalmitis patients and 11 controls were evaluated for GM and BDG using ELISA Kits. The Receiver Operating Characteristic (ROC) curves and diagnostic significance was calculated.

RESULTS

The median vitreous GM in culture-positive (60.83pg/ml) and culture-negative (59.9pg/ml) samples were higher than the (51.2pg/ml) control group. The median vitreous BDG in culture-positive (1.47pg/ml) and culture-negative (1.52pg/ml) samples were also similar, and higher than the control group (1.18pg/ml). ROC analysis showed that at a cut-off of 51.35pg/ml, the sensitivity and specificity for GM were 0.88 and 0.73.Similarly, for BDG at a cut-off of 1.18pg/ml, the sensitivity and specificity were 0.94 and 0.82 respectively.

CONCLUSION

Vitreous GM and BDG above the indicated threshold level could suggest a fungal infection, even when cultures are negative.

摘要

目的

评估玻璃体半乳甘露聚糖（GM）和 1,3 β-D-葡聚糖（BDG）水平在真菌性眼内炎诊断中的作用，重点关注培养阴性病例。

方法

使用 ELISA 试剂盒评估 31 例临床疑似真菌性眼内炎患者和 11 例对照者的玻璃体 GM 和 BDG。计算受试者工作特征（ROC）曲线和诊断意义。

结果

培养阳性（60.83pg/ml）和培养阴性（59.9pg/ml）样本的玻璃体 GM 中位数均高于对照组（51.2pg/ml）。培养阳性（1.47pg/ml）和培养阴性（1.52pg/ml）样本的玻璃体 BDG 中位数也较高，且高于对照组（1.18pg/ml）。ROC 分析表明，GM 的截止值为 51.35pg/ml 时，其敏感性和特异性分别为 0.88 和 0.73。同样，BDG 的截止值为 1.18pg/ml 时，敏感性和特异性分别为 0.94 和 0.82。