Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, PR China.
Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, PR China.
Ecotoxicol Environ Saf. 2022 May 1;236:113497. doi: 10.1016/j.ecoenv.2022.113497. Epub 2022 Apr 8.
Bioremediation, which has several advantages over traditional methods, represents an alternative means of dealing with heavy metal pollution. We screened for microorganisms showing heavy metal tolerance in polluted mangrove soils. A novel yeast, Geotrichum sp. CS-67, was discovered and tested for tolerance of Cu, Zn, and Ni. Zn was the most efficiently sequestered by Geotrichum sp. CS-67 followed by Ni and Cu. Zn and Ni were actively taken up into the cell, while Cu was adsorbed to the cell wall. We used RNA-Seq to show that a large number of genes involved in the physiological and biochemical processing of heavy metals were differentially expressed in this yeast when it was subjected to Zn and Ni stress. From this panel, we selected the SED1, GDI1 and ZRT1 genes for validation by qRT-PCR and discovered that, during Zn and Ni stress, SED1 and GDI1 were upregulated, while ZRT1 was downregulated, which was consistent with the RNA-Seq results and the biochemical function of these genes. In conclusion, the novel yeast Geotrichum sp. CS-67 has a marked ability to accumulate heavy metal ions, making it of great interest as a possible microbial agent for heavy metal pollution remediation in the future.
生物修复相对于传统方法具有许多优势，是处理重金属污染的一种替代方法。我们从污染红树林土壤中筛选出具有重金属耐受性的微生物。发现并测试了一种新型酵母，Geotrichum sp. CS-67，对 Cu、Zn 和 Ni 的耐受性。Zn 是 Geotrichum sp. CS-67 最有效地螯合的金属，其次是 Ni 和 Cu。Zn 和 Ni 被主动吸收到细胞内，而 Cu 被吸附到细胞壁上。我们使用 RNA-Seq 表明，当酵母受到 Zn 和 Ni 胁迫时，大量与重金属生理生化处理相关的基因表达差异。从这个面板中，我们选择了 SED1、GDI1 和 ZRT1 基因进行 qRT-PCR 验证，发现 SED1 和 GDI1 在 Zn 和 Ni 胁迫下上调，而 ZRT1 下调，这与 RNA-Seq 结果和这些基因的生化功能一致。总之，新型酵母 Geotrichum sp. CS-67 具有显著积累重金属离子的能力，因此有望成为未来重金属污染修复的潜在微生物制剂。
