Ramzan Muhammad Sajid, Rashid Muhammad Imran, Akbar Haroon, Avais Muhammad, Suleman Muhammad
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore 54200, Pakistan.
Department of Veterinary Medicine, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
Animals (Basel). 2022 Mar 23;12(7):813. doi: 10.3390/ani12070813.
Tropical theileriosis caused by the protozoan; is a tick-borne disease (TBD) transmitted by ticks of genus ; is clinically characterized by fever, anemia, and lymphadenopathy; and is responsible for heavy economic losses in terms of high morbidity and mortality rates with reduced production. Infected red blood cells of were inoculated into rabbits intraperitoneally, and propagation of has been investigated. The current study has shown an association between induced tropical theileriosis and variation of body temperature in rabbits. A significant rise in temperature (39.92 ± 0.33 °C) was seen on day 8 onwards, with the maximum temperature (40.27 ± 0.44 °C) on day 14 post-inoculation. In the current study, in vivo trials in susceptible cross-bred calves to investigate the attenuation and comparison with the infected group were also conducted. All the infected calves ( = 5) showed a significant rise in temperature (40.26 ± 0.05 °C) on day 10 onwards, with the maximum temperature (40.88 ± 0.05 °C) on day 16. The temperature of inoculated calves increased gradually post-inoculation, but the difference was not significant. A maximum parasitemia of 20% was observed in infected calves, but no piroplasm parasitemia was observed in inoculated calves. The prescapular lymph nodes of infected calves were enlarged, while the lymph nodes of inoculated calves remained normal throughout the trial. Analysis of clinical and parasitological responses of infected and inoculated calves showed a significant difference ( ≤ 0.05) in terms of temperature, parasitemia, and lymph node scoring between two groups. The current study was primarily aimed to attenuate in rabbit and to check its virulence in susceptible calves. It is concluded that propagation of in rabbits made it attenuated. Rabbit can be used as an in vivo model to weaken the virulence of .
由原生动物引起的热带泰勒虫病是一种由 属蜱传播的蜱传疾病（TBD）；其临床特征为发热、贫血和淋巴结病；并且由于高发病率、高死亡率以及产量下降而造成巨大经济损失。将感染的 红细胞腹腔内接种到兔子体内，并对 的繁殖情况进行了研究。当前研究表明，诱导的热带泰勒虫病与兔子体温变化之间存在关联。接种后第8天起体温显著升高（39.92 ± 0.33℃），接种后第14天达到最高温度（40.27 ± 0.44℃）。在当前研究中，还对易感杂交犊牛进行了体内试验，以研究其减毒情况并与感染组进行比较。所有感染的犊牛（n = 5）在第10天起体温显著升高（40.26 ± 0.05℃），第16天达到最高温度（40.88 ± 0.05℃）。接种犊牛的体温在接种后逐渐升高，但差异不显著。感染犊牛的最高寄生虫血症为20%，但接种犊牛未观察到梨形虫寄生虫血症。感染犊牛的肩胛前淋巴结肿大，而接种犊牛的淋巴结在整个试验过程中保持正常。对感染和接种犊牛的临床和寄生虫学反应分析表明，两组在体温、寄生虫血症和淋巴结评分方面存在显著差异（P≤ 0.05）。当前研究主要旨在使 在兔子体内减毒，并检查其在易感犊牛中的毒力。得出的结论是， 在兔子体内的繁殖使其减毒。兔子可作为一种体内模型来减弱 的毒力。
