Abrams E, Neigeborn L, Carlson M
Mol Cell Biol. 1986 Nov;6(11):3643-51. doi: 10.1128/mcb.6.11.3643-3651.1986.
The SNF2 and SNF5 genes are required for derepression of SUC2 and other glucose-repressible genes of Saccharomyces cerevisiae in response to glucose deprivation. Previous genetic evidence suggested that SNF2 and SNF5 have functionally related roles. We cloned both genes by complementation and showed that the cloned DNA was tightly linked to the corresponding chromosomal locus. Both genes in multiple copy complemented only the cognate snf mutation. The SNF2 gene encodes a 5.7-kilobase RNA, and the SNF5 gene encodes a 3-kilobase RNA. Both RNAs contained poly(A) and were present in low abundance. Neither was regulated by glucose repression, and the level of SNF2 RNA was not dependent on SNF5 function or vice versa. Disruption of either gene at its chromosomal locus still allowed low-level derepression of secreted invertase activity, suggesting that these genes are required for high-level expression but are not directly involved in regulation. Further evidence was the finding that snf2 and snf5 mutants failed to derepress acid phosphatase, which is not regulated by glucose repression. The SNF2 and SNF5 functions were required for derepression of SUC2 mRNA.
SNF2和SNF5基因是酿酒酵母中SUC2及其他葡萄糖可阻遏基因在葡萄糖缺乏时去阻遏所必需的。先前的遗传学证据表明，SNF2和SNF5具有功能相关的作用。我们通过互补作用克隆了这两个基因，并表明克隆的DNA与相应的染色体位点紧密连锁。多拷贝的这两个基因仅能互补同源的snf突变。SNF2基因编码一种5.7千碱基的RNA，而SNF5基因编码一种3千碱基的RNA。两种RNA都含有聚腺苷酸，且丰度较低。两者均不受葡萄糖阻遏的调控，并且SNF2 RNA的水平不依赖于SNF5的功能，反之亦然。在其染色体位点破坏任一基因仍可使分泌型转化酶活性有低水平的去阻遏，这表明这些基因是高水平表达所必需的，但不直接参与调控。进一步的证据是发现snf2和snf5突变体不能使酸性磷酸酶去阻遏，而酸性磷酸酶不受葡萄糖阻遏的调控。SUC2 mRNA的去阻遏需要SNF2和SNF5的功能。
