Department of Basic and Medical Genetics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin ave., 603022 Nizhny Novgorod, Russia.
Department of Neurotechnology, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin ave., 603022 Nizhny Novgorod, Russia.
Cells. 2022 Apr 4;11(7):1212. doi: 10.3390/cells11071212.
The current efforts in photodynamic therapy (PDT) of brain cancer are focused on the development of novel photosensitizers with improved photodynamic properties, targeted specific localization, and sensitivity to the irradiation dose, ensuring the effectiveness of PDT with fewer side effects for normal nerve tissue. Here, we characterize the effects of four photosensitizers of the tetracyanotetra(aryl)porphyrazine group (-) on the functional activity of neuron-glial networks in primary hippocampal cultures in their application in normal conditions and under PDT. The data revealed that the application of - leads to a significant decrease in the main parameters of the functional calcium activity of neuron-glial networks and pronounced changes in the network characteristics. The observed negative effects of - were aggravated under PDT. Considering the significant restructuring of the functional architectonics of neuron-glial networks that can lead to severe impairments in synaptic transmission and loss of brain functions, and the feasibility of direct application of PDT based on - in the therapy of brain tumors is highly controversial. Nevertheless, the unique properties of - retain a great prospect of their use in the therapy of tumors of another origin and cellular metabolism.
目前，脑癌光动力疗法（PDT）的研究重点是开发具有改进的光动力特性、靶向特定定位和对辐照剂量敏感的新型光敏剂，以确保 PDT 的有效性，同时减少对正常神经组织的副作用。在这里，我们研究了四种类四氰基四（芳基）卟啉基团的光敏剂（-）在正常条件下和 PDT 下应用于原代海马培养物中对神经元-神经胶质网络功能活性的影响。数据显示，-的应用导致神经元-神经胶质网络功能钙活性的主要参数显著降低，并导致网络特征明显变化。在 PDT 下，观察到的-的负性影响加剧。考虑到神经元-神经胶质网络的功能结构的显著重构可能导致突触传递严重受损和脑功能丧失，以及基于 - 直接应用于脑肿瘤治疗的可行性存在很大争议。然而，-的独特性质仍然使其在治疗其他来源的肿瘤和细胞代谢方面具有广阔的应用前景。
