• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

激酶抑制在体外缺血因子模型中的神经保护作用。

Neuroprotective Effect of Kinase Inhibition in Ischemic Factor Modeling In Vitro.

机构信息

Institute of Biology and Biomedicine, Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, 603950 Nizhny Novgorod, Russia.

Institute of Information, Technology, Mathematics and Mechanics, Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, 603950 Nizhny Novgorod, Russia.

出版信息

Int J Mol Sci. 2021 Feb 14;22(4):1885. doi: 10.3390/ijms22041885.

DOI:10.3390/ijms22041885
PMID:33672819
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7917718/
Abstract

The contribution of many neuronal kinases to the adaptation of nerve cells to ischemic damage and their effect on functional neural network activity has not yet been studied. The aim of this work is to study the role of the four kinases belonging to different metabolic cascades (SRC, Ikkb, eEF2K, and FLT4) in the adaptive potential of the neuron-glial network for modeling the key factors of ischemic damage. We carried out a comprehensive study on the effects of kinases blockade on the viability and network functional calcium activity of nerve cells under ischemic factor modeling in vitro. Ischemic factor modelling was performed on day 14 of culturing primary hippocampal cells obtained from mouse embryos (E18). The most significant neuroprotective effect was shown in the blockade of FLT4 kinase in the simulation of hypoxia. The studies performed revealed the role of FLT4 in the development of functional dysfunction in cerebrovascular accidents and created new opportunities for the study of this enzyme and its blockers in the formation of new therapeutic strategies.

摘要

许多神经元激酶对神经细胞适应缺血性损伤的贡献及其对功能性神经网络活动的影响尚未得到研究。本工作的目的是研究属于不同代谢级联的四种激酶（SRC、Ikkb、eEF2K 和 FLT4）在神经元-神经胶质网络适应潜能中的作用，以模拟缺血性损伤的关键因素。我们在体外缺血因子模型中对激酶阻断对神经细胞活力和网络功能钙活性的影响进行了全面研究。缺血因子模型是在培养的来自 E18 期小鼠胚胎的原代海马细胞第 14 天进行的。在模拟缺氧时，阻断 FLT4 激酶显示出最显著的神经保护作用。进行的研究揭示了 FLT4 在脑血管意外中功能障碍发展中的作用，并为研究该酶及其抑制剂在形成新的治疗策略中的作用创造了新的机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/1c5be6f3a529/ijms-22-01885-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/2273143a66b8/ijms-22-01885-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/52acd73f6efe/ijms-22-01885-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/13c9fbc107a0/ijms-22-01885-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/6a7a62592955/ijms-22-01885-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/eb61f65bc5f6/ijms-22-01885-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/d1ba02171ae4/ijms-22-01885-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/c0bd2d3714b9/ijms-22-01885-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/f35befc91c1f/ijms-22-01885-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/1c5be6f3a529/ijms-22-01885-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/2273143a66b8/ijms-22-01885-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/52acd73f6efe/ijms-22-01885-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/13c9fbc107a0/ijms-22-01885-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/6a7a62592955/ijms-22-01885-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/eb61f65bc5f6/ijms-22-01885-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/d1ba02171ae4/ijms-22-01885-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/c0bd2d3714b9/ijms-22-01885-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/f35befc91c1f/ijms-22-01885-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5cc9/7917718/1c5be6f3a529/ijms-22-01885-g009.jpg

相似文献

1
Neuroprotective Effect of Kinase Inhibition in Ischemic Factor Modeling In Vitro.激酶抑制在体外缺血因子模型中的神经保护作用。
Int J Mol Sci. 2021 Feb 14;22(4):1885. doi: 10.3390/ijms22041885.
2
Intracellular Neuroprotective Mechanisms in Neuron-Glial Networks Mediated by Glial Cell Line-Derived Neurotrophic Factor.胶质细胞源性神经营养因子介导的神经元-神经胶质网络中的细胞内神经保护机制。
Oxid Med Cell Longev. 2019 Nov 18;2019:1036907. doi: 10.1155/2019/1036907. eCollection 2019.
3
Vascular endothelial growth factor: direct neuroprotective effect in in vitro ischemia.血管内皮生长因子：在体外缺血中的直接神经保护作用
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10242-7. doi: 10.1073/pnas.97.18.10242.
4
Inhibition of Neuronal Necroptosis Mediated by RIPK1 Provides Neuroprotective Effects on Hypoxia and Ischemia In Vitro and In Vivo.抑制 RIPK1 介导的神经元坏死性凋亡对体外和体内缺氧缺血具有神经保护作用。
Int J Mol Sci. 2022 Jan 10;23(2):735. doi: 10.3390/ijms23020735.
5
eEF2K inhibition blocks Aβ42 neurotoxicity by promoting an NRF2 antioxidant response.eEF2K 抑制通过促进 NRF2 抗氧化反应来阻断 Aβ42 的神经毒性。
Acta Neuropathol. 2017 Jan;133(1):101-119. doi: 10.1007/s00401-016-1634-1. Epub 2016 Oct 17.
6
Double-Edged Sword of Vitamin D3 Effects on Primary Neuronal Cultures in Hypoxic States.维生素 D3 对缺氧状态下原代神经元培养的双重影响。
Int J Mol Sci. 2021 May 21;22(11):5417. doi: 10.3390/ijms22115417.
7
Propofol protects hippocampal neurons from apoptosis in ischemic brain injury by increasing GLT-1 expression and inhibiting the activation of NMDAR via the JNK/Akt signaling pathway.丙泊酚通过增加谷氨酸转运体-1（GLT-1）的表达并经由JNK/Akt信号通路抑制N-甲基-D-天冬氨酸受体（NMDAR）的激活，从而保护海马神经元免受缺血性脑损伤中的细胞凋亡。
Int J Mol Med. 2016 Sep;38(3):943-50. doi: 10.3892/ijmm.2016.2663. Epub 2016 Jul 5.
8
Glial cell line-derived neurotrophic factor (GDNF) counteracts hypoxic damage to hippocampal neural network function in vitro.胶质细胞系源性神经营养因子（GDNF）在体外可对抗海马神经网络功能的缺氧损伤。
Brain Res. 2018 Jan 1;1678:310-321. doi: 10.1016/j.brainres.2017.10.023. Epub 2017 Nov 8.
9
Clenbuterol protects mouse cerebral cortex and rat hippocampus from ischemic damage and attenuates glutamate neurotoxicity in cultured hippocampal neurons by induction of NGF.克仑特罗可保护小鼠大脑皮层和大鼠海马免受缺血性损伤，并通过诱导神经生长因子减轻培养海马神经元中的谷氨酸神经毒性。
Brain Res. 1996 Apr 22;717(1-2):44-54. doi: 10.1016/0006-8993(95)01567-1.
10
A network-based method for mechanistic investigation and neuroprotective effect on treatment of tanshinone Ⅰ against ischemic stroke in mouse.一种基于网络的方法用于对丹参酮Ⅰ治疗小鼠缺血性中风的作用机制进行研究及神经保护作用评估
J Ethnopharmacol. 2021 May 23;272:113923. doi: 10.1016/j.jep.2021.113923. Epub 2021 Feb 20.

引用本文的文献

1
Impact of 5-HT4 Receptors on Neuron-Glial Network Activity In Vitro.5-羟色胺4受体对体外神经元-神经胶质网络活动的影响
Int J Mol Sci. 2025 Aug 9;26(16):7718. doi: 10.3390/ijms26167718.
2
Unravelling Contributions of Astrocytic Connexin 43 to the Functional Activity of Brain Neuron-Glial Networks under Hypoxic State In Vitro.揭示星形胶质细胞连接蛋白43在体外缺氧状态下对脑神经元-胶质细胞网络功能活动的贡献。
Membranes (Basel). 2022 Sep 28;12(10):948. doi: 10.3390/membranes12100948.
3
3D-printed hyaluronic acid hydrogel scaffolds impregnated with neurotrophic factors (BDNF, GDNF) for post-traumatic brain tissue reconstruction.

本文引用的文献

1
M2 microglial small extracellular vesicles reduce glial scar formation the miR-124/STAT3 pathway after ischemic stroke in mice.M2 小胶质细胞外囊泡通过 miR-124/STAT3 通路减少缺血性脑卒中后小鼠的神经胶质瘢痕形成。
Theranostics. 2021 Jan 1;11(3):1232-1248. doi: 10.7150/thno.48761. eCollection 2021.
2
Avenanthramide C Prevents Neuronal Apoptosis via PI3K/Akt/GSK3β Signaling Pathway Following Middle Cerebral Artery Occlusion.芹菜素酰胺C通过大脑中动脉闭塞后PI3K/Akt/GSK3β信号通路预防神经元凋亡。
Brain Sci. 2020 Nov 20;10(11):878. doi: 10.3390/brainsci10110878.
3
Excitotoxicity: Still Hammering the Ischemic Brain in 2020.
3D打印的含有神经营养因子（脑源性神经营养因子、胶质细胞源性神经营养因子）的透明质酸水凝胶支架用于创伤后脑组织重建。
Front Bioeng Biotechnol. 2022 Aug 25;10:895406. doi: 10.3389/fbioe.2022.895406. eCollection 2022.
4
Unraveling of Functional Activity of Primary Hippocampal Neuron-Glial Networks in Photodynamic Therapy Based on Tetracyanotetra(aryl)porphyrazines.基于四氰基四（芳基）卟啉的光动力学疗法中初级海马神经元-神经胶质网络功能活动的解析。
Cells. 2022 Apr 4;11(7):1212. doi: 10.3390/cells11071212.
5
Inhibition of Neuronal Necroptosis Mediated by RIPK1 Provides Neuroprotective Effects on Hypoxia and Ischemia In Vitro and In Vivo.抑制 RIPK1 介导的神经元坏死性凋亡对体外和体内缺氧缺血具有神经保护作用。
Int J Mol Sci. 2022 Jan 10;23(2):735. doi: 10.3390/ijms23020735.
兴奋毒性：2020年仍在重创缺血性脑
Front Neurosci. 2020 Oct 26;14:579953. doi: 10.3389/fnins.2020.579953. eCollection 2020.
4
Signatures of the Consolidated Response of Astrocytes to Ischemic Factors In Vitro.体外缺血因素对星形胶质细胞综合反应的特征。
Int J Mol Sci. 2020 Oct 26;21(21):7952. doi: 10.3390/ijms21217952.
5
Activated microglia-derived macrophage-like cells exacerbate brain edema after ischemic stroke correlate with astrocytic expression of aquaporin-4 and interleukin-1 alpha release.缺血性脑卒中后激活的小胶质细胞衍生的巨噬细胞样细胞加重脑水肿与星形胶质细胞表达水通道蛋白-4和白细胞介素-1α释放相关。
Neurochem Int. 2020 Nov;140:104848. doi: 10.1016/j.neuint.2020.104848. Epub 2020 Sep 11.
6
Neuroprotective Effect of HIF Prolyl Hydroxylase Inhibition in an In Vitro Hypoxia Model.缺氧诱导因子脯氨酰羟化酶抑制在体外缺氧模型中的神经保护作用
Antioxidants (Basel). 2020 Jul 24;9(8):662. doi: 10.3390/antiox9080662.
7
Neuroprotective effects of astaxanthin against oxygen and glucose deprivation damage via the PI3K/Akt/GSK3β/Nrf2 signalling pathway in vitro.虾青素通过体外 PI3K/Akt/GSK3β/Nrf2 信号通路对氧葡萄糖剥夺损伤的神经保护作用。
J Cell Mol Med. 2020 Aug;24(16):8977-8985. doi: 10.1111/jcmm.15531. Epub 2020 Jun 21.
8
Brain-to-cervical lymph node signaling after stroke.脑向颈淋巴结的卒中后信号传递。
Nat Commun. 2019 Nov 22;10(1):5306. doi: 10.1038/s41467-019-13324-w.
9
Brain expression of the vascular endothelial growth factor gene family in cognitive aging and alzheimer's disease.血管内皮生长因子基因家族在认知衰老和阿尔茨海默病中的脑表达
Mol Psychiatry. 2021 Mar;26(3):888-896. doi: 10.1038/s41380-019-0458-5. Epub 2019 Jul 22.
10
Role of DJ-1 in the mechanism of pathogenesis of Parkinson's disease.DJ-1 在帕金森病发病机制中的作用。
J Bioenerg Biomembr. 2019 Jun;51(3):175-188. doi: 10.1007/s10863-019-09798-4. Epub 2019 May 3.